Optimizing Platelet Transfusion for Clinical Practice. Do Platelets's Age Matter for Therapeutic Platelets Transfusion? | ||||
| The Medical Journal of Cairo University | ||||
| Volume 91, Issue 12, December 2023, Page 1601-1606 PDF (600.56 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2024.342764 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| MOHAMMED SHARIF, M.Sc.*; BARAA NOORWALI, M.Sc.*; FOUAD AL ZAHRANI, M.Sc.*; AHMED SALAMI, M.Sc.*; TAHANI AL ZAHRANI, M.Sc.*; ZAINAH BUKHARI, M.Sc.*;; WAFA AL ANAZZI, M.Sc.*; HAWAZEN SULIMAN, M.Sc.*; MAJED ALGARNI, M.Sc.*; OSAMA AL ZAHARNI, M.Sc.*; SAMAH MOKHLIS, M.Sc.*; AMANI GUSTI, M.D.; SAMY ATTALLAH, M.D.** * | ||||
| The Departments of Clinical Pathology, Hematology, King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia* and Faculty of Medicine, Mansura University** | ||||
| Abstract | ||||
| Abstract Background: Platelet concentrates (PCs) with the imple-mentation of bacterial testing have a storage period of 7 days. It was reported that platelet function decreased gradually in vitro until the 5th or 7th day of storage. Pooled PCs (PPCs) are main-ly used in Europe and Canada and there is the possibility to use many PPCs with 7 days storage for transfusion in case of bleeding or massive transfusion. Aim of Study: The aim of this study is to test in vitro both storage conditions and function of platelet-rich plasma (PRP-PCs) during storage for 7 days to assess the function of the PCs for the possibility to extend the use of stored PCs up to 7 days and its use for therapeutic and massive platelets transfusion. Patients and Methods: In this study 18 PRP-PCs, were evaluated for chemical analysis by measuring pH, LDH pro-duction, and glucose consumption levels and platelet aggre-gation testing using adenosine diphosphate (ADP), collagen (COLL), and ristocetin (RIST) and epinephrine (EPI) on days 1,3,5, and 7 of storage of PCs. Results: The platelets yield was >5.58 x 1010 which indi-cates accurate platelet separation and storage. The metabolic parameters indicated stable storage conditions throughout the 7-day period. The chemical analysis showed that there is stable pH during all days of storage above 6.4. Aggregation respons-es of the PCs decreased gradually and significantly (p<0.001) starting from 1st day when compared with 3rd, 5th and 7th day. On the 7th day the platelets lost their response to EPI and ADP, while still maintaining a normal response to RIST and weak to COLL. Conclusion: There is a loss of some function on the 6-7th day of platelet storage which may indicate that it is suitable for prophylactic more than therapeutic transfusion. In order to pro-vide more clinical benefit to the patients with thrombocytopen-ic bleeding, the platelets should be less than 7 days of storage. | ||||
| Keywords | ||||
| Platelets transfusion — Platelets concentrate —Platelets function | ||||
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