Evaluation of miR-181a in Diabetes Mellitus Type-2 and its relation to Diabetic Nephropathy
Hussain, A., AbdelAziz, G., Othman, A., Tohamy, M., Abdel-Tawab, M., Reyad, H. (2024). Evaluation of miR-181a in Diabetes Mellitus Type-2 and its relation to Diabetic Nephropathy. EKB Journal Management System, 5(1), 116-132. doi: 10.21608/ejmr.2024.250377.1497
Ahmad Adel Hussain; Ghada Mahmoud AbdelAziz; Asmaa Mohamed Othman; Mervat Abdel-Baseer Tohamy; Marwa Sayed Abdel-Tawab; Hoda Ramadan Reyad. "Evaluation of miR-181a in Diabetes Mellitus Type-2 and its relation to Diabetic Nephropathy". EKB Journal Management System, 5, 1, 2024, 116-132. doi: 10.21608/ejmr.2024.250377.1497
Hussain, A., AbdelAziz, G., Othman, A., Tohamy, M., Abdel-Tawab, M., Reyad, H. (2024). 'Evaluation of miR-181a in Diabetes Mellitus Type-2 and its relation to Diabetic Nephropathy', EKB Journal Management System, 5(1), pp. 116-132. doi: 10.21608/ejmr.2024.250377.1497
Hussain, A., AbdelAziz, G., Othman, A., Tohamy, M., Abdel-Tawab, M., Reyad, H. Evaluation of miR-181a in Diabetes Mellitus Type-2 and its relation to Diabetic Nephropathy. EKB Journal Management System, 2024; 5(1): 116-132. doi: 10.21608/ejmr.2024.250377.1497

Evaluation of miR-181a in Diabetes Mellitus Type-2 and its relation to Diabetic Nephropathy

Egyptian Journal of Medical Research
Volume 5, Issue 1, January 2024, Page 116-132  XML PDF (546.42 K)
Document Type: Original Article
DOI: 10.21608/ejmr.2024.250377.1497
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Authors
Ahmad Adel Hussain email 1; Ghada Mahmoud AbdelAziz1; Asmaa Mohamed Othman2; Mervat Abdel-Baseer Tohamy3; Marwa Sayed Abdel-Tawab1; Hoda Ramadan Reyad1
1Medical Biochemistry Department, Faculty of Medicine, Beni-Suef University, Egypt
2Internal Medicine Department, Faculty of Medicine, Beni-Suef University, Egypt
3Medical Microbiology and Immunology Department, Faculty of Medicine, Beni-Suef University, Egypt.
Abstract
A chronic consequence of diabetes mellitus (DM), diabetic nephropathy (DN) typically advances to end-stage renal disease (ESRD). However, the pathophysiology of DN is not fully understood. It has been revealed that several miRNAs have been considered as risk or protective factors in DN.  Inflammation is a key determining factor in the development and progression of diabetes. The aim of the current study was to investigate the association of serum miR-181a, serum, and urine IL-6, and IL- 18 with DN. miR-181a was estimated by RT- qRT-PCR, IL-6, and IL- 18 were estimated by enzyme-linked immunosorbent assay (ELISA). 120 patients were divided into three groups; Group 1: 40 DM type 2 without DN, group 2: 40 DM type 2 with diabetic DN, and group 3: 40 control patients. Group 2 was further subdivided into 20 macroalbuminuria and 20 microalbuminuria patients. When DN patients were compared to other groups, their levels of miR-181a, IL-6, and IL-18 increased significantly. Higher levels of miR-181a in patients with macroalbuminuria than patients with microalbuminuria in DN group. Serum miR-181a significantly positively correlated with fasting insulin, fasting glucose, HOMA IR, ACR, serum IL-6 and BMI in DN group. The ROC curve analysis revealed high significant specificity and sensitivity of serum miR-181a, serum, and urine IL-6, and IL-18 suggesting their possible utility for the primary diagnosis of DN in DM. It is concluded that miR-181a may prove to be a valuable biomarker for DN prognostic and diagnostic applications
Keywords
miR-181a; IL-6; IL-18; diabetes mellitus; and diabetic nephropathy
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