Examining The impact of Chitosan and Nano-Chitosan Addition on The expression Levels of SOD and CAT Genes in Two Lines of Japanese Quail

Ramadan, Gomaa Said; Abd El-Maged, Marwa Hosni; Yassein, Doaa M.M.; El-Tarras, Mai A; Abdel-Halim, hassan A.H.; Abdelsalam, Ahmed Mosaad; Abdel-Halim, Amal A

doi:10.21608/ejvs.2024.256140.1731

	Examining The impact of Chitosan and Nano-Chitosan Addition on The expression Levels of SOD and CAT Genes in Two Lines of Japanese Quail
Egyptian Journal of Veterinary Sciences
Volume 55, Issue 6, November and December 2024, Page 1637-1648 PDF (1.89 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.256140.1731
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Authors
Gomaa Said Ramadan ¹; Marwa Hosni Abd El-Maged²; Doaa M.M. Yassein²; Mai A El-Tarras³; hassan A.H. Abdel-Halim²; Ahmed Mosaad Abdelsalam ⁴; Amal A Abdel-Halim ⁵
¹National Research Centre, Egypt
²Animal production Research Institute
³Cairo University, Fac. of Agriculture
⁴National Research Centre
⁵Department of Animal Production, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt
Abstract
This research aims to investigate the antioxidative potential of dietary chitosan (CH) and nano-chitosan (NCH) supplementation on the gene expression of pivotal antioxidant defense system components, namely SOD (superoxide dismutase) and CAT (catalase) genes. These genes are instrumental in safeguarding cells against the deleterious effects of reactive oxygen species. The study extends its scope to evaluating the hepatic and intestinal histology in two distinct lines of Japanese quail. Carried out as a fully randomized design experiment with 10 treatment groups, the research encompassed 840 one-day-old Japanese quails from two distinct lines: Sq, selected for body weight at 30 days after 42 generations of selection, and Jq, a control line without selection. Each line was further divided into five treatment groups, with three replicates per group and 28 unsexed chicks per replicate. The treatment groups included a baseline diet according to NRC (1994) guidelines, two groups supplemented with chitosan (CH) at 50 and 70 mg/kg diet, and two groups supplemented with nano-chitosan (NCH) at 30 and 50 mg/kg diet for both Sq and Jq lines. The study outcomes shed light on the varying concentrations of chitosan (30, 50, and 70 mg/kg) and their impacts on live body weight, CAT and SOD gene expression levels, as well as histopathological changes in the liver and intestine. The findings revealed discernible effects across all tested concentrations, elucidating the multifaceted influence of chitosan and nano-chitosan supplementation on the examined parameters.
Keywords
CAT; Chitosan (CH); Japanese quail; Nano-chitosan (NCH); SOD


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