Kaempferol Motivative Effects on Mitochondrial Antioxidant Function and Intrinsic Antioxidant Enzymes of Buffalo Oocytes (Part-II) | ||||
| Benha Veterinary Medical Journal | ||||
| Volume 45, Issue 2, December 2023, Page 46-51 PDF (292.46 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2023.233455.1717 | ||||
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| Authors | ||||
Hager Saeed Bahgat1; Alaa Abdelghafar2; Magdy Ramadan Badr3; Basant Mohamed Nagib Shafik4; Rasha El Sayed Azab 5; Sherif Ibrahim mohamed Ramadan 4; Mohamed El-Raey Mohamed  6
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| 1Department of Theriogenology, Faculty of Veterinary Medicine, Benha University. | ||||
| 2Theriogenology department -benha university- faculty of veterinary medicine. | ||||
| 31) Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research Center, Al Haram, Giza, Egypt. | ||||
| 4Animal Wealth Development Department, Faculty of Veterinary Medicine, Benha University. | ||||
| 5Department of Physiology, Faculty of Veterinary Medicine, Benha University, | ||||
| 6Theriogenology, Faculty of Veterinary Medicine , Benha University | ||||
| Abstract | ||||
| Many of Kaempferol’s biological functions have been widely investigated, but its role in animal reproduction still needs great attention and exploration, especially in assisted reproductive techniques due to its promising mechanisms in modulating oxidative stress. The current study aimed to explore its roles in maintaining the redox hemostasis of buffalo oocytes that matured in vitro in the presence of different Kaempferol concentrations (0,5,10 and 15 µg/ml). The current study revealed for the first time that Kaempferol at 10 µg/ml has great capability to scavenge the generated free radicals such as H2O2 (26.4±2.27 ng/ml), and nitric oxide (25.9±2.63 nmol/L). Moreover, it significantly attenuates the rate of lipid peroxidation (1.77±0.17 nmol/L) in buffalo oocytes matured in vitro. Firstly, by enhancing the scavenging mechanisms. Secondly, by increasing the intrinsic antioxidants enzymes synthesis such as SOD (33.70±2.96 U/ml), and GSH (5.51±0.37 nmol/l). Additionally, the current results revealed that Kaempferol can protect the oocyte mitochondria from apoptosis by increasing the intra-mitochondrial antioxidants such as SOD (4.46±0.21 nmol/mg) which prevents mitochondrial dysfunction. In conclusion, Kaempferol’s addition to the in vitro maturation media of buffalo oocytes significantly ameliorates its developmental competence by increasing its quality and developmental potentials as a whole, controlling the redox hemostasis by scavenging actions as well as by enhancing the production of intrinsic antioxidant enzymes, and finally by protecting the mitochondrial functions. | ||||
| Keywords | ||||
| Kaempferol; Buffalo; Oocyte; Antioxidant; Mitochondria | ||||
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