Biochemical and histopathological changes of L-Arginine experimentally induced acute pancreatitis in male albino rats.
Disoukey, M., Fararh, K., Farid, A., Gad, F. (2023). Biochemical and histopathological changes of L-Arginine experimentally induced acute pancreatitis in male albino rats.. EKB Journal Management System, 45(2), 77-81. doi: 10.21608/bvmj.2023.248680.1752
Marwa Azab Disoukey; Khalid Fararh; Ayman Farid; Fatma Gad. "Biochemical and histopathological changes of L-Arginine experimentally induced acute pancreatitis in male albino rats.". EKB Journal Management System, 45, 2, 2023, 77-81. doi: 10.21608/bvmj.2023.248680.1752
Disoukey, M., Fararh, K., Farid, A., Gad, F. (2023). 'Biochemical and histopathological changes of L-Arginine experimentally induced acute pancreatitis in male albino rats.', EKB Journal Management System, 45(2), pp. 77-81. doi: 10.21608/bvmj.2023.248680.1752
Disoukey, M., Fararh, K., Farid, A., Gad, F. Biochemical and histopathological changes of L-Arginine experimentally induced acute pancreatitis in male albino rats.. EKB Journal Management System, 2023; 45(2): 77-81. doi: 10.21608/bvmj.2023.248680.1752

Biochemical and histopathological changes of L-Arginine experimentally induced acute pancreatitis in male albino rats.

Benha Veterinary Medical Journal
Volume 45, Issue 2, December 2023, Page 77-81  XML PDF (405.82 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.248680.1752
View on SCiNiTO View on SCiNiTO
Authors
Marwa Azab Disoukey email ; Khalid Fararh email ; Ayman Farid email orcid ; Fatma Gad email
Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Egypt
Abstract
The biochemical and histopathological changes of L-arginine experimentally induced acute pancreatitis (AP) in male albino rats was investigated. Forty male albino rats were divided into two groups (20 rats each). Group I (control group): rats were fed normal diet. Group II (Acute Pancreatitis Group (AP): Acute pancreatitis was induced in male albino rats by injecting 300 mg/kg bwt of L –Arginine (Arg) intraperitoneally , as a 10% solution in 0.15 M NaCl .Blood samples were collected after 6, 24 and 96 hours and 7 days for acute group schedules. Collected serum samples were directly used for determination of amylase, lipase, alanine aminotransferase (ALT) and aspartate aminotransferase (AST ) activities beside glucose level also tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6 ),nitric oxide (NO),total cholesterol ,triglyceride ,urea and creatinine levels were determined . The obtained results showed significant increases in serum amylase , lipase, ALT and AST activities as well as cholesterol, TNF-α , IL-6 . NO , serum Urea and Creatinine levels. Meanwhile, non-significant change in plasma glucose and serum triglycerides levels were observed. Histopathological changes of L-arginine treated group showed, 24 hours post administration, focal acinar necrosis characterized by pyknosis of nuclei and deeply stained cytoplasm with retention of acinar architecture compared with control group.
Keywords
: L-arginine; Acute pancreatitis; exocrine pancreatic enzymes; pro inflammatory cytokines
Statistics
Article View: 42
PDF Download: 34