Assessment of Biofilm Production in E. coli Isolated from Broilers Farm Water Systems
Abdel razek, N., Abd El-Tawab, A., Hassan, H. (2023). Assessment of Biofilm Production in E. coli Isolated from Broilers Farm Water Systems. EKB Journal Management System, 45(2), 137-140. doi: 10.21608/bvmj.2023.235090.1720
Nouran Mostafa Abdel razek; Ashraf Abd El-Tawab; Heba Hassan. "Assessment of Biofilm Production in E. coli Isolated from Broilers Farm Water Systems". EKB Journal Management System, 45, 2, 2023, 137-140. doi: 10.21608/bvmj.2023.235090.1720
Abdel razek, N., Abd El-Tawab, A., Hassan, H. (2023). 'Assessment of Biofilm Production in E. coli Isolated from Broilers Farm Water Systems', EKB Journal Management System, 45(2), pp. 137-140. doi: 10.21608/bvmj.2023.235090.1720
Abdel razek, N., Abd El-Tawab, A., Hassan, H. Assessment of Biofilm Production in E. coli Isolated from Broilers Farm Water Systems. EKB Journal Management System, 2023; 45(2): 137-140. doi: 10.21608/bvmj.2023.235090.1720

Assessment of Biofilm Production in E. coli Isolated from Broilers Farm Water Systems

Benha Veterinary Medical Journal
Volume 45, Issue 2, December 2023, Page 137-140  XML PDF (339.78 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.235090.1720
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Authors
Nouran Mostafa Abdel razek email orcid 1; Ashraf Abd El-Tawab2; Heba Hassan3
11 Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt. 2 Animal Health Research Institute, Agriculture Research Center, Dokki, P.O. Box 246, Giza 12618, Egypt.
2Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt
3National Research Centre, El-Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Abstract
The E. coli biofilm is made up of a bacterial colony that is immersed in an extracellular polymeric substance (EPS) matrix that shields the microorganisms from harmful environmental factors and causes infection. The development of antibiotic resistance exacerbates the diversity in structural elements of biofilm, making it more difficult to eradicate. The present work review focuses on a variety of E. coli issues. So, the study aim is biofilm production evaluation in E. coli isolated from the broiler water system.In this study, one hundred swabs were collected from drinking water pipes and drinkers from different broiler farms in Giza and Dakahlia governorates in Egypt. The swabs were examined for the presence of E. coli and their ability for biofilm production. Fifty swabs were positive for E. coli after biochemical and serological identification of isolates. Microtiter-plate test for determination of biofilm production showed that forty isolates produce biofilm with different degrees, classified 13 (26%) strong biofilms, 17(34%) intermediate biofilms and 10(20%) weak biofilms,10 isolates out of 50 (20%) exhibited no biofilm. The PCR detection of the adrA gene in E. coli isolates originating from broiler environments was performed, specifically focusing on the thirteen strong biofilm-forming isolates.
Keywords
E. coli; Biofilm; Broilers; adrA gene; Water system
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