GENETIC DIVERSITY ANALYSIS OF SOME EGYPTIAN RICE VARIETIES DIFFERING IN STORAGE ABILITY BASED ON SSR MARKERS | ||||
| Egyptian Journal of Plant Breeding | ||||
| Volume 23, Issue 4, March and April 2019, Page 483-499 PDF (572.53 K) | ||||
| Document Type: Original research | ||||
| DOI: 10.12816/ejpb.2019.344251 | ||||
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| Abstract | ||||
| This investigation was conducted at Sakha Agricultural Research Station during the period from 1st November 2014 to 31st October 2016 to evaluate some Egyptian rice cultivars belonging to different rice types under different storage conditions (room temperature, cooling and freezing) for 24 months with different temperature degrees. Eleven Egyptian rice cultivars with 18 SSR markers related to storage ability were used in order to determine the genetic diversity and polymorphism among the studied genotypes. Six SSR markers, namely RM1, RM5, RM71, RM251, RM161 and RM234 showed high polymorphism among the studied rice cultivars, and more-over differentiate them to different groups based on their storage ability. A total of 21 polymorphic alleles were detected and ranged between two by RM234 and five by RM71 markers. Based on the data obtained from the SSR markers, the results showed that the phylogenetic analysis divided the studied accessions into two main groups (A and B) in addition to some sub groups belong to each main group. Group A included 7 rice cultivars related to japonica rice type. On the other hand, group B included 4 cultivars divided into two sub groups (indica and indica/japonica types). The japonica cultivars were affected by storage duration more than the others, particularly for germination % and germination index under two years storage conditions. High temperature increased the negative storage effects. Indica and indica / japonica cultivars were found to be tolerant to storage more than japonica cultivars and keep more seed viability with storage time. According to the seed germination and viability after 24 months of storage, 5 Co was considered the most appropriate storage condition for short-term storage of rice seeds. | ||||
| Keywords | ||||
| Rice; Genetic diversity; Storage ability; SSR | ||||
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