Blood Eosinophils as a Predictor of Acute Attacks of Asthma in Children | ||||
Minia Journal of Medical Research | ||||
Volume 33, Issue 3, July 2022, Page 84-88 PDF (185.87 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/mjmr.2024.145555.1093 | ||||
View on SCiNiTO | ||||
Authors | ||||
Asmaa Natag Reyad1; Hend M Moness2; Eman Nady Hassan 3; Marwa Waly ELdin Ali4 | ||||
1Assistant Professor of pediatrics, Faculty of medicine, Minia University | ||||
2Department of Clinical Pathology, Faculty of Medicine, Minia University, El-Minia, Egypt. | ||||
3Department of Pediatrics, Faculty of Medicine, Minia University, El-Minia, Egypt. | ||||
4pediatric department,Faculty of Medicine, Minia University | ||||
Abstract | ||||
Childhood asthma represents a heterogeneous challenging disease, particularly in its severe forms. The identification of different asthma phenotypes has stimulated research in underlying molecular mechanisms, such as the endotypes, and paved the way to the search for related specific biomarkers, which may guide diagnosis, management and prediction of treatment response The present study was a cross-sectional hospital based study carried out at pediatric departments, Minia University.It was conducted on 60 children known as asthmatic, they were attended to Minia University Hospital, their age were from 3 years to 12 years. They were divided into 2 groups: group I: It was included 30 asthmatic children presented to the pediatric department with asthma exacerbation according to GINA 2021, group II: It was included 30 stable asthmatic children. Results: There was a statistically significant higher values of (TLC, eosinophil count) detected in the exacerbation group than stable group with p value (0.008, <0.001) respectively Blood eosinophils have significant moderate positive correlation with number of exacerbation (R=0.58, p value= <0.001), blood eosinophils have significant moderate negative correlation with control status (R=-0.58, p value= <0.001) there is highest values of blood eosinophils in uncontrolled groups (p value <0.001). Blood Eosinophils is a potent predictor marker for asthma exacerbation (OR=9.699) Conclusion: We determined that previous history of severe exacerbation, blood eosinophil counts was associated with the risk of future exacerbation in severe asthma despite receiving multiple therapy. Eosinophils are involved in the pathogenesis of asthma exacerbation and reportedly associated with the frequency of asthma exacerbation. | ||||
Keywords | ||||
Childhood asthma; Blood Eosinophils; hematological biomarker | ||||
Full Text | ||||
Introduction Asthma is a chronic heterogeneous disease of the lower airways characterized by chronic inflammation and airway hyper-reactivity leading to cough, wheeze, diffi-culty in breathing, and chest tightness.[1]
Severe asthma is a relatively uncommon condition in children but one which causes morbidity, occasionally mortality, and is a challenging condition to manage. There are several definitions of severe asthma, which have a common theme of poor control despite high dose inhaled corticosteroid treatment.
Severe asthma is defined by the European Respiratory Society/American Thoracic Society (ERS/ATS) criteria as either asthma requiring escalation to step 5 medical therapy (=high-dose ICS in combination with a second controller and/or additional systemic corticosteroid therapy) to maintain asthma control or asthma that remains uncontrolled despite step 5 therapy.[2, 3].
Eosinophils are involved in the development of asthma exacerbation, Eosinophils, which tend to accumulate at sites of allergic inflammation, contribute to the development of bronchial asthma. They release a number of mediators, including s radical oxygen species, cytokines, such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-8, and lipid mediators, such as cysteinyl leukotrienes (cysLTs) [4]. the role of eosinophils in the development of airway remodeling has been established at a relatively early phase. Eosinophil-deficient mice are reportedly protected from peribronchiolar collagen deposition. Eosinophils produce trans-forming growth factor (TGF)-β, which may contribute to airway fibrosis. Additionally, eosinophils can produce cysLTs and be a major cellular source of cysLTs in the airways of individuals with seasonal allergic asthma or aspirin-exacerbated respiratory disease, which also contribute to airway remodeling. Anti-IL-5 mAb suppresses airway remodeling (reduction of tenascin, lumican, and procollagen III) as well as airway eosinophils expressing mRNA for TGF-β1 and concentrations of TGF-β1 in the bronchoalveolar lavage (BAL) fluid of asthmatics[4].
Patients and methods The present study was a cross-sectional hospital-based study carried out at Pediatric Departments of Minia University. It was conducted on 60 children known as asthmatic, they were attended to Minia University Hospital, their age range was (3 -12) years.
They were divided into 2 groups: Group I: It was included 30 asthmatic children presented to the pediatric department with asthma exacerbation according to GINA 2021, Group II: It was included 30 stable asthmatic children.
All subjects included in the study were subjected to the following:
Considering age, sex, age of first attack of wheezes, age of diagnosis, last exacerbation, severity, controller therapy, family history of asthma, history of atopic dermatitis or allergic rhinitis.
Through general and chest examination. * General examination: including vital data, general systemic examination to exclude other chronic diseases. * Complete chest examination: inspection, palpation, percussion and auscultation. 3) Laboratory investigation: CBC, blood eosinophils count.
Results In this study the exacerbation group includes 30 children, their age range (3-12) years, 23 of them were males and 7of them were females, and they diagnosed to have asthma (3±0.6) years ago and their age of 1st wheezes was (1-3) years, while the controlled asthma group includes 30 children, their age range (3-12) years, 8 0f them were males and 22 of them were females, and they diagnosed to have asthma (3.1±0.6) years ago and their age of 1st wheezes was (1.4-3) years. (table 1)
In this study there was a statistically significant higher values of (TLC, eosinophil count) detected in the asthma exacerbation group than stable asthmatic group with p value (0.008, <0.001) respectively. Table (2)
Blood eosinophils have significant moderate positive correlation with number of exacerbation (R=0.58, p value= <0.001), blood eosinophils have significant moderate negative correlation with control status (R=-0.58, p value= <0.001). Table (3), while the highest values of blood eosinophils were recorded in uncontrolled asthmatic group (p value <0.001). Table (4). The most potent predictor marker for asthma exacerbation is blood eosinophils (OR=9.699) Table (5) Discussion Childhood asthma represents a hetero-geneous challenging disease, particularly in its severe forms. The identification of different asthma phenotypes has stimulated research in underlying molecular mechanisms, such as the endotypes, and paved the way to the search for related specific biomarkers, which may guide diagnosis, management, and predict response to treatment [5].
Some patients with severe asthma experience acute attacks despite receiving multiple therapy. The risk of acute attacks and heterogeneous response to treatment may be associated with specific inflam-matory molecules that are responsive or resistant to corticosteroids[6].
Biomarker analysis may help in tailoring treatment and predicting the future risk of exacerbation in patients with severe asthma [7-9].
As regard the value of blood biomarkers in both groups, we found that TLC had higher values in group I (exacerbation group) than group II (stable group) p value 0.008. In agreement with result done by Rabah et al., who found that highTLC associated with asthma exacerbation and reflect disease severity.
We found significant higher values of eosinophils in group I (exacerbation group) than group II (stable group), p value <0.001 and by simple logistic regression analysis of blood biomarkers for prediction of asthma exacerbation, eosinophils were the most potent predictor for exacerbation (OR=9.6, CI=3.3-28.4, P value <0.001). These findings were the same as a study done by Nakagome and Nagata who found that blood eosinophil counts are important factors for predicting asthma exacerbation, moreover we found that highest values of eosinophils in uncontrolled patients. in agreement with this results study was Choi et al., who found that blood eosinophilia is associated with more severe symptoms and lower response to anti-inflammatory medications.
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References | ||||
Conclusion
We determined that previous history of severe-to-serious exacerbation, blood eosinophil counts was associated with the risk of future exacerbation in severe asthma despite receiving multiple therapy. Eosinophils are involved in the patho-genesis of asthma exacerbation and it is associated with the frequency of asthma exacerbation.
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