Anticancer Activity of Cytosine Deaminase Enzyme Purified From Local Saccharomyces cerevisia Bread Yeast | ||||
| Journal of Bioscience and Applied Research | ||||
| Articles in Press, Accepted Manuscript, Available Online from 06 March 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2024.268103.1036 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Zainab Yaseen Mohammed Hasan  1; Muna Abed Mutter2; Milad Adnan Mezher3
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| 1Biotechnology Research Center-Al-Nahrain university | ||||
| 2Collage of Education for Pure Sciences University Tikrit, Salah al-din , Iraq | ||||
| 3Biology,collage of Education for pure Sciences,University of Tikrit, Salah l-din.Iraq | ||||
| Abstract | ||||
| The study aimed to extract and purify the enzyme cytosine deaminase from locally manufactured bread yeast Saccharomyces cerevisia. To achieve the goal of the study, bread yeast was made locally and transported to the laboratory for enzyme extraction process using toluene as organic solvent for yeast wall rapturing. Crude enzyme extracted with cold distilled water. Steps for enzyme purification starting by precipitation step with ammonium sulfate 60% saturation, then ion exchange purification method, ended with gel filtration purification proses had been applied. In each step, the supernatant volume, enzyme activity, specific enzyme activity, protein concentration, and percentage of enzyme yield had been recorded. The cytotoxic effect of different enzyme concentrations in all purification steps toward the lung cancer cell line A549 and human breast cancer line MDA cell line beside normal cell line REF cells was investigated. The specific activity of the crude extract of locally produced bread yeast reached 13.205 units/mg protein, and for ammonium sulfate enzyme precipitation the specific activity was amounted 17.68 units/mg protein, for ion exchange was 216.66 units/mg protein, while for purification with gel filtration the specific activity reached 571.428 unit/mg protein. The cytotoxic effect of the extracted enzyme in all steps; crud, ion-exchange, and gel filtration purified enzyme on the lungA549 and breast AMD cancer lines beside the REF normal cells were should a range of toxic effect at different enzyme concentration. The toxicity was reduced till diminishing as the enzyme was more purified. | ||||
| Keywords | ||||
| cytosine deaminase; A549 cell line; MDA cell line; Saccharomyces cerevisia | ||||
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