CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP. | ||||
| Menoufia Journal of Agricultural Biotechnology | ||||
| Article 3, Volume 9, Issue 2, February 2024, Page 23-38 PDF (1.17 MB) | ||||
| Document Type: original papers | ||||
| DOI: 10.21608/mjab.2024.271332.1016 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Muhammad ELsobky   1; Khalid Salah Abdel-lateif2; Abdelmegid Fahmi1; Abdelfatah Elzanaty3; Ragaa Eissa1
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| 1Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt | ||||
| 2Department of Genetics, Faculty of Agriculture, Menoufia University | ||||
| 3Department of Poultry and Fish Production Faculty of Agriculture, Menoufia University, Shibin El-Kom | ||||
| Abstract | ||||
| : Twenty-six isolates used in qualitative screening of Cellulase done in two media CMC-Congo red dye plate and 1% PASC (phosphoric acid swollen cellulose) plate. Quantitative Enzymes activities were tested in two fermentation methods SSF (solid state fermentation) and SmF (Submerged fermentation). SmF was detected at 2,3,4,6 and 7 days in Mandel’s Media with CMC 0.5%. Moreover, SSF was carried out by using straws (Rice and wheat) for all isolates. The cellulase activities measured as (FPase, CMCase and β-glucosidase). In this study positive correlation coefficient in enzymes activity between SSF and qualitative screening in (CMC & PASC) plate. Higher correlation have been detected between PACS and SSF rice straw was about (0.595**) for β-Glucosidase. After all these tests we can compare enzyme production by SmF and SSF. Data showed SmF production for high yield and high quality. The cellulase genes, Chb gene encoding cellobiohydrolase 2 (CBH2) and Bgl gene that encoding endo-β-gluconase (BGL1) were present in all Trichoderma isolates. | ||||
| Keywords | ||||
| Trichoderma Spp; Cellulase genes; SSF; SmF; CMCase; FPase; β; glucosidase; CBH2; BGL1 | ||||
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