CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP. | ||
| Menoufia Journal of Agricultural Biotechnology | ||
| Article 3, Volume 9, Issue 2, February 2024, Pages 23-38 PDF (1.35 M) | ||
| Document Type: original papers | ||
| DOI: 10.21608/mjab.2024.271332.1016 | ||
| Authors | ||
| Muhammad ELsobky* 1; Khalid Salah Abdel-lateif2; Abdelmegid Fahmi1; Abdelfatah Elzanaty3; Ragaa Eissa1 | ||
| 1Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt | ||
| 2Department of Genetics, Faculty of Agriculture, Menoufia University | ||
| 3Genetics Department Faculty of agriculture Menoufia Univesity Shibin El kom | ||
| Abstract | ||
| : Twenty-six isolates used in qualitative screening of Cellulase done in two media CMC-Congo red dye plate and 1% PASC (phosphoric acid swollen cellulose) plate. Quantitative Enzymes activities were tested in two fermentation methods SSF (solid state fermentation) and SmF (Submerged fermentation). SmF was detected at 2,3,4,6 and 7 days in Mandel’s Media with CMC 0.5%. Moreover, SSF was carried out by using straws (Rice and wheat) for all isolates. The cellulase activities measured as (FPase, CMCase and β-glucosidase). In this study positive correlation coefficient in enzymes activity between SSF and qualitative screening in (CMC & PASC) plate. Higher correlation have been detected between PACS and SSF rice straw was about (0.595**) for β-Glucosidase. After all these tests we can compare enzyme production by SmF and SSF. Data showed SmF production for high yield and high quality. The cellulase genes, Chb gene encoding cellobiohydrolase 2 (CBH2) and Bgl gene that encoding endo-β-gluconase (BGL1) were present in all Trichoderma isolates. | ||
| Keywords | ||
| Trichoderma Spp; Cellulase genes; SSF; SmF; CMCase; FPase; β; glucosidase; CBH2; BGL1 | ||
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