Role of miR-155 expression in acute myeloid leukemia and correlation with nucleophosmin 1 (NPM1) mutations modyfied | ||
| ARCADEs of MEDICINE | ||
| Volume 2, Issue 1, August 2024, Pages 1-7 PDF (360.83 K) | ||
| Document Type: Original Research | ||
| DOI: 10.21608/arcmed.2024.270038.1045 | ||
| Authors | ||
| Laila A Hegazy1; Nohair Soliman1; Aml S Nasr* 2; Omnia Y Abd El Dayem1; Hanan R Nassar3; Reem M Embaby4 | ||
| 1Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt. | ||
| 2Kasr Al Aini School of medicine,Cairo,Egypt | ||
| 3Department of medical oncology , National cancer institute , Cairo University, Egypt. | ||
| 4National nutrition institute | ||
| Abstract | ||
| Background: Acute myeloid leukemia (AML) is a highly heterogeneous disease marked by the inability of progenitor cells in the bone marrow to differentiate terminally from developing to mature blood cells . Furthermore, microRNAs (miRNAs) are short, non-coding RNAs that regulate multiple gene expressions that regulate hematopoietic stem/progenitor cell commitment and differentiation. Methods: The present study involved 40 patients newly diagnosed with AML in addition to 40 matched controls. A quantitative reverse transcription real-time PCR (qRT-PCR) assay was performed to quantify miR-155. The NPM1 mutations in a new cohort of Egyptian AML patients were assessed using RT-PCR assays. The aim was to examine the correlation between these mutations and microRNAs, as well as to determine their expression in relation to demographic, clinical, and laboratory data. Results: For Mir-155, there was were a statistically substantial differences between the mean CT values of patients and controls. NPM1 results exhibited marked differences between controls and patients. Conclusion: In conclusion, the value of 155 did not accurately predict cases of AML. Additionally, no correlation was observed between NPM1 and miR-155. | ||
| Keywords | ||
| AML; miR-155; qRT-PCR; NMP | ||
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