DEVELOPMENT OF RESISTANCE TO FOUR INSECTICIDES AND ENHANCED DETOXIFYING ENZYME ACTIVITIES IN THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)
Ammar, H., El-Bermawy, Z., El-Sheikh, T., Sayedahmed, M., Abouelghar, G. (2024). DEVELOPMENT OF RESISTANCE TO FOUR INSECTICIDES AND ENHANCED DETOXIFYING ENZYME ACTIVITIES IN THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.). EKB Journal Management System, 9(2), 99-114. doi: 10.21608/mjapam.2024.268032.1032
Hager A. Ammar; Zeinab Abd El-ghany El-Bermawy; Tarek El-Sheikh; Mahmoud Abd Elaleem Sayedahmed; Gamal E Abouelghar. "DEVELOPMENT OF RESISTANCE TO FOUR INSECTICIDES AND ENHANCED DETOXIFYING ENZYME ACTIVITIES IN THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)". EKB Journal Management System, 9, 2, 2024, 99-114. doi: 10.21608/mjapam.2024.268032.1032
Ammar, H., El-Bermawy, Z., El-Sheikh, T., Sayedahmed, M., Abouelghar, G. (2024). 'DEVELOPMENT OF RESISTANCE TO FOUR INSECTICIDES AND ENHANCED DETOXIFYING ENZYME ACTIVITIES IN THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)', EKB Journal Management System, 9(2), pp. 99-114. doi: 10.21608/mjapam.2024.268032.1032
Ammar, H., El-Bermawy, Z., El-Sheikh, T., Sayedahmed, M., Abouelghar, G. DEVELOPMENT OF RESISTANCE TO FOUR INSECTICIDES AND ENHANCED DETOXIFYING ENZYME ACTIVITIES IN THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.). EKB Journal Management System, 2024; 9(2): 99-114. doi: 10.21608/mjapam.2024.268032.1032

DEVELOPMENT OF RESISTANCE TO FOUR INSECTICIDES AND ENHANCED DETOXIFYING ENZYME ACTIVITIES IN THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)

Menoufia Journal of Plant Protection
Article 9, Volume 9, Issue 2, February 2024, Page 99-114  XML PDF (749.49 K)
Document Type: Original Article
DOI: 10.21608/mjapam.2024.268032.1032
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Authors
Hager A. Ammar email 1; Zeinab Abd El-ghany El-Bermawy2; Tarek El-Sheikh3; Mahmoud Abd Elaleem Sayedahmed4; Gamal E Abouelgharorcid 5
1faculty of agriculture
2Department of pesticides, faculty of agriculture, menoufia university
3Plant Protection Research Institute, Agricultural Research Center
4PhD student
5Moustafa Kamel Street
Abstract
: The Egyptian cotton leafworm Spodoptera littoralis (Bosid.), one of the main pests in Egypt that causes damage to industrial, vegetable, and ornamental crops. The development of resistance and biochemical mechanism of S. littoralis to four insecticides (deltamethrin, spinetoram, pyridalyl and indoxacarb) were studied in the laboratory. Selection pressure in all experiments was carried out on 4th instar larvae for six generations by the leaf dipping technique. At the end of selection, the results indicated that the resistance ratios (RR) were 43.79-, 29.69-, 13.09- and 33.39-fold for deltamethrin, spinetoram, pyridalyl and indoxacarb, respectively, compared with the parent strain. At the end of selection pressure, detoxifying enzyme assays revealed that the α-esterases activity levels for such insecticides were 5542.64, 4024.44, 4223.64 and 3641.32, respectively compared with parent strain 447.89, whereas those of β-esterase activity were 4132.30, 2343.25, 2953.70 and 1294.04 for deltamethrin, spinetoram, pyridalyl and indoxacarb, respectively, higher than in the parent strain 400.61. In addition, there was a significant increase in levels of AChE, ACP, ALP and GSH activity at the end of selection with these insecticides in all selected generations. The results demonstrated that, treatment of S. littoralis with DMT, SPT, PYD and INC compared with the susceptible strain caused a significant decrease in the protein, carbohydrate and lipid contents.
Keywords
Insecticides; Resistance; Enzymatic activity; Cotton Leafworm; Spodoptera littoralis
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