Pharmacopeial constants, secondary metabolites and antibacterial activity of calli induced in vitro from flaxseed sprouts explants
Hussein, E., Abd El-Sadek, M., Abd El-Hakem, A., Ismail, A., Aref, M. (2023). Pharmacopeial constants, secondary metabolites and antibacterial activity of calli induced in vitro from flaxseed sprouts explants. EKB Journal Management System, 2(2), 202-208. doi: 10.21608/ijtar.2023.202633.1047
Esam A Hussein; Marwa A Abd El-Sadek; Abd El-Hakem S Abd El-Hakem; Ahmed M Ismail; Mohamed S Aref. "Pharmacopeial constants, secondary metabolites and antibacterial activity of calli induced in vitro from flaxseed sprouts explants". EKB Journal Management System, 2, 2, 2023, 202-208. doi: 10.21608/ijtar.2023.202633.1047
Hussein, E., Abd El-Sadek, M., Abd El-Hakem, A., Ismail, A., Aref, M. (2023). 'Pharmacopeial constants, secondary metabolites and antibacterial activity of calli induced in vitro from flaxseed sprouts explants', EKB Journal Management System, 2(2), pp. 202-208. doi: 10.21608/ijtar.2023.202633.1047
Hussein, E., Abd El-Sadek, M., Abd El-Hakem, A., Ismail, A., Aref, M. Pharmacopeial constants, secondary metabolites and antibacterial activity of calli induced in vitro from flaxseed sprouts explants. EKB Journal Management System, 2023; 2(2): 202-208. doi: 10.21608/ijtar.2023.202633.1047

Pharmacopeial constants, secondary metabolites and antibacterial activity of calli induced in vitro from flaxseed sprouts explants

International Journal of Theoretical and Applied Research
Article 5, Volume 2, Issue 2, December 2023, Page 202-208  XML PDF (389 K)
Document Type: Original Article
DOI: 10.21608/ijtar.2023.202633.1047
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Authors
Esam A Hussein1; Marwa A Abd El-Sadek email orcid 2; Abd El-Hakem S Abd El-Hakemorcid 3; Ahmed M Ismailorcid 4; Mohamed S Areforcid 5
1Azhar university
2jasmeen 2, 5th settelment
3Botany and microbiology Department, Faculty of Science, Al-Azhar University
4Botany and microbiology Department, Faculty of Science, Al-Azhar university.
5Botany and microbiology Department, Faculty of Science, Al-Azhar University.
Abstract
In the present study, some important pharmaceutical compounds in flaxseed plant have been done via biotechnology techniques. the seeds of Linum usitatissimum were surface sterilized germinated for one week on hormone-free MS culture media for 10 days. Cotyledonary leaves, hypocotyls segments (1 cm) and rootlets segments (1 cm) of the obtained sprouts were used as explants for callus induction on MS supplemented with BA (benzyladenine) and NAA (Naphthalene acetic acid). Maximum callus fresh weight was recorded in from rootlets explants and reached up to 3.72 gram in response to the treatment with 0.5 mg/l BA plus 0.25 mg/l NAA after 4 weeks of treatment. The highest callus fresh weight from cotyledonary leaves and hypocotyls segments were recorded in response to the treatment with 0.75 mg/l BA plus 0. 5 mg/l NAA and 0. 5 mg/l BA and NAA, respectively. The results obtained have revealed many differences between the resulted calli with respect to pharmacopeial constants and secondary metabolites determined (Phenolics, glycosides, flavonoids and saponins). Methanolic extracts of calli obtained from different explants exhibited antibacterial activity against both the Gram-positive and Gram-negative tested pathogenic bacterial strains. The maximum inhibition zone (26 mm) was obtained in response to the treatment of staphylococcus aureus with extract of callus obtained from cotyledonary leaf explants. This study may indicate that the callus of L. usitatissimum can be adopted in the future as a source of valuable metabolites.
Keywords
In vitro; Linum usitatissimum; Plant biotechnology; Plant tissue culture
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