Royal Jelly and Green Tea Effect on P53 Upregulated Modulator of Apoptosis (PUMA) Gene and TNF Gene
Abdel-Mageed, W., Fathy, R., Othman, M. (2016). Royal Jelly and Green Tea Effect on P53 Upregulated Modulator of Apoptosis (PUMA) Gene and TNF Gene. EKB Journal Management System, 2(Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology), 1-10. doi: 10.21608/rjab.2016.34674
Wael Abdel-Mageed; Rasha Fathy; Mohamed Othman. "Royal Jelly and Green Tea Effect on P53 Upregulated Modulator of Apoptosis (PUMA) Gene and TNF Gene". EKB Journal Management System, 2, Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology, 2016, 1-10. doi: 10.21608/rjab.2016.34674
Abdel-Mageed, W., Fathy, R., Othman, M. (2016). 'Royal Jelly and Green Tea Effect on P53 Upregulated Modulator of Apoptosis (PUMA) Gene and TNF Gene', EKB Journal Management System, 2(Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology), pp. 1-10. doi: 10.21608/rjab.2016.34674
Abdel-Mageed, W., Fathy, R., Othman, M. Royal Jelly and Green Tea Effect on P53 Upregulated Modulator of Apoptosis (PUMA) Gene and TNF Gene. EKB Journal Management System, 2016; 2(Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology): 1-10. doi: 10.21608/rjab.2016.34674

Royal Jelly and Green Tea Effect on P53 Upregulated Modulator of Apoptosis (PUMA) Gene and TNF Gene

Research Journal of Applied Biotechnology
Article 1, Volume 2, Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology, June 2016, Page 1-10  XML PDF (733.2 K)
Document Type: Original Article
DOI: 10.21608/rjab.2016.34674
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Authors
Wael Abdel-Mageed email 1; Rasha Fathy2; Mohamed Othman3
1molecular Biology Dept., Genetic Engineering & Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Sadat City, Egypt
2Molecular Biology Dept., Genetic Engineering & Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Sadat City, Egypt.
3Bioinformatics Dept., Genetic Engineering & Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Sadat City, Egypt.
Abstract
The purpose of this study examine whether the polymorphism in (TNF-α) gene correlated with activate p53 upregulated modulator of apoptosis (PUMA) gene consequential exposure to pesticides and the effects of green tea and royal jelly in rats. Experimental groups were organized into 12 groups inclucing 10 animals per each. Contain control group, pesticide group and the rest of groups were pesticide + protective. Blood samples were collected from rats before and after occupational exposure to pesticides for liver and kidney enzymes biochemical analysis, the polymorphism of the genes or TNF-α and PUMA were typed from genomicDNA and Allelic frequencies and carriage ates were comparedbetween healthy and treated rats. The correlation between TNF-α and PUMA genotypes and the clinical characteristics of rats were also evaluated. Data statistical analysis for mitochomdrial enzymes in our study showed a very highly significan (P<0.001) difference in the mean value of serum enzumes isolated from treatments groups with pesticides before and after Royal jelly and green tea treatments. The results of restriction enzymes for TNF-α showed there are nol different between negative control with and both of two protective agents in the results of restricted bands. But the results from PUMA showed a strong deferent between the treatments with royal jelly and green tea combined with pesticides. The royal jelly and green tea enhance the repair system enzymes to remedy the mutation that originatedin DNA after exposure to pesticides. Our recent work provides direct evidence Royal jelly and green tea could be affects on mechanism related to the repair system in cell to remedy the DNA mutation. 
Keywords
royal jelly; green tea; p53; TNF gene
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