The antibiogram of multidrug-resistant Pseudomonas aeruginosa with oprL gene associated with surgical site infections in Bauchi, Nigeria | ||||
Microbes and Infectious Diseases | ||||
Articles in Press, Accepted Manuscript, Available Online from 23 March 2024 | ||||
Document Type: Original Article | ||||
DOI: 10.21608/mid.2024.261574.1752 | ||||
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Authors | ||||
Titus Onyi 1, 2; Hassan Muhammad DOKO 2; ELIJAH ELLA2 | ||||
1Department of Community Medicine, Abubakar Tafawa Balewa University, Bauchi, Nigeria | ||||
2Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria | ||||
Abstract | ||||
Aim: The objectives of the study were first to isolate, characterize, and detect oprL gene in Pseudomonas aeruginosa isolates associated with surgical site infections (SSIs). The second objective was to conduct drug susceptibility and resistance assays of the Pseudomonas aeruginosa isolates with the oprL genes. Materials and Methods: The study was a cross-sectional hospital-based study that analyzed 250 post-surgical wound swabs from patients(n=250) in two hospitals in Bauchi, Nigeria for the presence of Pseudomonas aeruginosa. After the analyses of bacterial colonial morphology on Cetrimide agar, Gram Staining, biochemical characterization, and PCR assay with oprL as the target gene, the antibiogram was conducted with 10 antibiotics from various classes using the Kirby Bauer Disc Diffusion method. Results: Five out of the 250 swabs were positive for Pseudomonas aeruginosa from the series of assays- a prevalence of 2.0%.All 5 isolates had the oprL gene, with an amplicon size of 504bp. The antibiogram reported Multiple Drug Resistance(MDR) in 80% of the isolates. The Multiple Antibiotic Resistance(MAR) indices ranged from 0.2 to 0.5. All the isolates were susceptible to Ofloxacin and all resistant to Cotrimoxazole. Conclusion: All the isolates had the oprL gene, further confirming the specificity of this virulence factor for detecting Pseudomonas aeruginosa. The virulence of the oprL gene reflects the multidrug resistanceand high MAR indices of the isolates. The study recommends surveillance antibiograms for Pseudomonas aeruginosa with the oprL gene associated with SSIs, to optimize the treatment of SSIs. | ||||
Keywords | ||||
: Multidrug-resistance; Pseudomonas aeruginosa; Surgical Site Infections; oprL gene; Polymerase Chain Reaction | ||||
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