Genetic and Antigenic Relatedness of Highly Pathogenic Avian Influenza H5Nx Viruses Currently Circulating in Egypt and Some H5 Commercial Vaccines
Hamouda, A., Sultan, H., Ibrahim, M., Abd Elhafez, A. (2019). Genetic and Antigenic Relatedness of Highly Pathogenic Avian Influenza H5Nx Viruses Currently Circulating in Egypt and Some H5 Commercial Vaccines. EKB Journal Management System, 1(1), 60-74. doi: 10.21608/jcvr.2019.10168.1000
Amira Hamdy Hamouda; Hesham Sultan; Mahmoud Ibrahim; Awad Abd Elhafez. "Genetic and Antigenic Relatedness of Highly Pathogenic Avian Influenza H5Nx Viruses Currently Circulating in Egypt and Some H5 Commercial Vaccines". EKB Journal Management System, 1, 1, 2019, 60-74. doi: 10.21608/jcvr.2019.10168.1000
Hamouda, A., Sultan, H., Ibrahim, M., Abd Elhafez, A. (2019). 'Genetic and Antigenic Relatedness of Highly Pathogenic Avian Influenza H5Nx Viruses Currently Circulating in Egypt and Some H5 Commercial Vaccines', EKB Journal Management System, 1(1), pp. 60-74. doi: 10.21608/jcvr.2019.10168.1000
Hamouda, A., Sultan, H., Ibrahim, M., Abd Elhafez, A. Genetic and Antigenic Relatedness of Highly Pathogenic Avian Influenza H5Nx Viruses Currently Circulating in Egypt and Some H5 Commercial Vaccines. EKB Journal Management System, 2019; 1(1): 60-74. doi: 10.21608/jcvr.2019.10168.1000

Genetic and Antigenic Relatedness of Highly Pathogenic Avian Influenza H5Nx Viruses Currently Circulating in Egypt and Some H5 Commercial Vaccines

Journal of Current Veterinary Research
Article 8, Volume 1, Issue 1, June 2019, Page 60-74  XML PDF (387.52 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2019.10168.1000
View on SCiNiTO View on SCiNiTO
Authors
Amira Hamdy Hamouda email 1; Hesham Sultan2; Mahmoud Ibrahim3; Awad Abd Elhafez4
1poultry and rabbit medicine department, faculty of veterinary medicine, Sadat city university, Egypt
2Department of Birds and Rabbits Medicine, Faculty of Veterinary Medicine, University of Sadat City, Menoufiya, Egypt
3Birds and Rabbits Medicine department, Faculty of Veterinary Medicine, Sadat City University, Menoufia, Egypt
4poultry diseases department, Faculty of veterinary medicine, Assuit University, Assuit, Egypt
Abstract
In the present study, samples were collected from wild and domestic waterfowls to detect recently circulating HPAI H5Nx viruses. Partial HA gene sequencing followed by phylogenetic analysis of the obtained sequences of five selected isolates was performed. Phylogenetic analysis revealed clustering of the three H5N8 isolates in clade 2.3.4.4b, with other Egyptian H5N8 isolates with amino acid identity percent with each other ranging from (96.9% - 99.3%). The two H5N1 isolates clustered in clade 2.2.1.2 with other recent Egyptian isolates. The amino acid identity percent between vaccinal strains was ranging from (88.2% - 89.6%) with H5N8 field isolates and (96.5% - 99%) with H5N1 field isolates. The amino acid identity percent between H5N8 and H5N1 field isolates ranging from (86.3% - 87.8%). The antigenic relatedness between the two HPAI local isolates (H5N1 and H5N8) was low (R value 24.8%) indicating major antigenic difference between the two subtypes. Also, a major subtype difference in antigenicity (R value 20.7-31.0%) was also detected between the local HPAI H5N8 isolate and the three vaccinal strains. While, a minor subtype difference (R value 38.7-68.7%) was detected among the examined H5N1 local isolate and the three vaccinal strains. Our results support that, HPAI H5N8 virus is genetically and antigenically distinct from HPAI H5N1 virus. Also, some commercial vaccines which are used to control avian influenza in Egypt are genetically and antigenically distinct from HPAI H5N8 virus currently circulating in Egypt.
Keywords
Antigenic; Egypt; Genetic; HPAI; H5Nx
Statistics
Article View: 633
PDF Download: 591