The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies
Elbatanony, M., Aly, H., Deabes, D., El-Sayed, A., Younis, E. (2024). The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies. EKB Journal Management System, 67(10), 341-359. doi: 10.21608/ejchem.2024.273155.9393
Marwa M. Elbatanony; Hanan F. Aly; Doaa Abd Elhady Deabes; Ahmed F. El-Sayed; Eman A. Younis. "The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies". EKB Journal Management System, 67, 10, 2024, 341-359. doi: 10.21608/ejchem.2024.273155.9393
Elbatanony, M., Aly, H., Deabes, D., El-Sayed, A., Younis, E. (2024). 'The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies', EKB Journal Management System, 67(10), pp. 341-359. doi: 10.21608/ejchem.2024.273155.9393
Elbatanony, M., Aly, H., Deabes, D., El-Sayed, A., Younis, E. The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies. EKB Journal Management System, 2024; 67(10): 341-359. doi: 10.21608/ejchem.2024.273155.9393

The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies

Egyptian Journal of Chemistry
Volume 67, Issue 10, October 2024, Page 341-359  XML PDF (1.57 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.273155.9393
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Authors
Marwa M. Elbatanony email orcid 1; Hanan F. Alyorcid 2; Doaa Abd Elhady Deabes1; Ahmed F. El-Sayedorcid 3, 4; Eman A. Younisorcid 2
1Pharmacognosy Department, National Research Centre (NRC), El Behouth St., P.O. 12622, Cairo, Egypt.
2Therapeutic Chemistry Department, National Research Centre (NRC), 33 El Buhouth St., P.O. 12622, Cairo, Egypt.
3Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt.
4Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt.
Abstract
The study targets anti-hypothyroditic effect and phytochemistry of the Ficus carica L. leaves pet. ether extract. GC/MS data of the unsaponifiable fraction led to identification of 23 compounds (85.14%) while identified saponifiable fraction amounted 78.56%. TLC chromatography led to isolation of five triterpenes (α-amyrin, taraxerol, lupeol, 3, 22 dihydroxy-13 (18) oleanene and β- sitosterol). The extract exhibited promising in vitro anti-inflammatory response via fluorometric assay. The extract was orally given at a dose (500 mg/kg) where different biomarkers were measured in vivo: serum TSH, T3, T4, IL-6 and oxidative stress levels. Ficus carica pet. ether extract recorded amelioration in all parameters suggesting its significance against thyroid hypothyroidism induced in male rats as prophylaxis and treatment. Histological examinations of all the groups supported the results. Additionally, molecular docking studies reinforced the results, demonstrating that the major compounds exhibited high binding interactions with (COX-1), (5-LOX), (IL-6), and thyroid stimulating hormone receptor.
Keywords
Anti-hypothyroidism; Ficus carica; GC/MS; triterpenes; TSH; IL-6; molecular docking
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