Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens
Draz, S., Bayoumi, A., Shaapan, R., Zidan, S., Toaleb, N., Hadad, G. (2025). Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens. EKB Journal Management System, 56(1), 21-32. doi: 10.21608/ejvs.2024.275492.1899
Sara H. Draz; Ahmed Bayoumi; Raafat Mohamed Shaapan; Shrif A. Zidan; Nagwa Ibrahim Toaleb; Ghada A. Hadad. "Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens". EKB Journal Management System, 56, 1, 2025, 21-32. doi: 10.21608/ejvs.2024.275492.1899
Draz, S., Bayoumi, A., Shaapan, R., Zidan, S., Toaleb, N., Hadad, G. (2025). 'Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens', EKB Journal Management System, 56(1), pp. 21-32. doi: 10.21608/ejvs.2024.275492.1899
Draz, S., Bayoumi, A., Shaapan, R., Zidan, S., Toaleb, N., Hadad, G. Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens. EKB Journal Management System, 2025; 56(1): 21-32. doi: 10.21608/ejvs.2024.275492.1899

Sero-immunological Investigation of Cystic Echinococcosis: Comparison and Evaluation of Diagnostic Performances of Six Hydatid Cyst Antigens

Egyptian Journal of Veterinary Sciences
Volume 56, Issue 1, January 2025, Page 21-32  XML PDF (966.87 K)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.275492.1899
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Authors
Sara H. Draz1; Ahmed Bayoumi2; Raafat Mohamed Shaapan email orcid 1; Shrif A. Zidanorcid 2; Nagwa Ibrahim Toaleborcid 3; Ghada A. Hadad2
1Dept. of Zoonotic Diseases, Veterinary Research Division, National Research Centre
2Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, University of Sadat City, Egypt.
3Prof of Parasitic immunology, Veterinary Research Division
Abstract
This study compares and assesses the efficacy of various hydatid cyst antigens for the accurate immunological diagnosis of CE in both humans and camels. Outer layer (Ol.C) germinal layer (Gl.C) fluid (Fl.C) and protoscolices (Ps.C) antigens prepared from camel hydatid cyst. In addition, fluid (Fl.H) and protoscolices (Ps.H) prepared from human hydatid cyst aspirate. The all six antigen types investigated as immunodiagnostic tools in human and camel hydatidosis using ELISA, SDS-PAGE and western blot assays. ELISA sowed higher binding reactivity by protoscolices antigens (Ps.C and Ps.H) followed by fluid antigens (Fl.C and Fl.H), while outer and germinal layer antigens (Ol.C and Gl.C) give the lower activates. Ps.C antigen exhibiting higher sensitivity (100%), than Ps.H antigen (84.6 and 85.3%) against both positive camels and human sera. The sero-prevalence of Cystic echinococcosis was 59.78% and 43.65% in examined camel and human sera, respectively using the diagnostic Ps.C antigen. According to SDS-PAGE, all six hydatid cyst antigens demonstrated significant electrophoretic similarity, particularly in three bands of 42, 36, and 22 kDa, Using immunoblotting techniques, all separated antigens identified a sharing in two common bands with the same molecular weights of 42 a and 22 kDa. The obtained results concluded that camel and human hydatid cyst protoscolices antigens are introduced in this investigation as an effective diagnostic antigen and new immune-reactive fractions of 42 and 22 kDa for the diagnosis of hydatidosis in humans and camels.
Keywords
Hydatid cyst antigens; Camel; Human; ELISA; SDS and immune blotting
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