Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections
Ahmad, N., Wamidh, Q., Abdulrahman, T. (2024). Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections. EKB Journal Management System, 10(1), 85-102. doi: 10.21608/jbaar.2024.348053
Noor Ahmad; Qudus Wamidh; Thanaa Rasheed Abdulrahman. "Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections". EKB Journal Management System, 10, 1, 2024, 85-102. doi: 10.21608/jbaar.2024.348053
Ahmad, N., Wamidh, Q., Abdulrahman, T. (2024). 'Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections', EKB Journal Management System, 10(1), pp. 85-102. doi: 10.21608/jbaar.2024.348053
Ahmad, N., Wamidh, Q., Abdulrahman, T. Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections. EKB Journal Management System, 2024; 10(1): 85-102. doi: 10.21608/jbaar.2024.348053

Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections

Journal of Bioscience and Applied Research
Volume 10, Issue 1, March 2024, Page 85-102  XML PDF (1.45 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2024.348053
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Authors
Noor Ahmad email 1; Qudus Wamidh2; Thanaa Rasheed Abdulrahman2
1Department Microbiology, Medical College/Al Nahrain University, Iraq
2Department Microbiology Medical College/Al Nahrain University, Iraq
Abstract
Background: Urinary tract infections (UTIs) frequently affect the urinary system, predominantly caused by Escherichia coli (E. coli) and Klebsiella pneumoniae. This study examines the prevalence of these bacteria and bla OXA-48 and bla IMP genes. Objectives: The research aims to assess the occurrence of MDR and XDR E. coli and K. pneumoniae in pediatric UTI cases, including the resistance genes bla OXA-48 and bla IMP. Methods: A total of 100 pediatric UTI urine samples were analyzed for antibiotic resistance, ESBL detection, and bla OXA-48 and bla IMP gene presence using molecular techniques, gene sequencing, and statistical analysis via SPSS software. Results: High resistance rates to common antibiotics were found in K. pneumoniae and E. coli isolates. bla OXA-48 was detected in 92.3% of E. coli and 100% of K. pneumoniae isolates, while bla IMP was found in 43.1% of E. coli and 25.7% of K. pneumoniae isolates. No nucleic acid variation was observed in the blaIMP-1 sequences of both bacteria.
    Conclusion: The significant presence of bla OXA-48 and bla IMP genes in K. pneumoniae and E. coli highlights the urgent need for enhanced surveillance and careful antibiotic use to curb antibiotic-resistant UTIs in children.Bottom of Form
 
Keywords
bla OXA-48 gene; bla IMP gene; Urinary tract infections; E. coli; Klebsiella pneumoniae
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