Evaluation of green synthesized silver nanoparticles utilizing freeze dried curcumin nanocrystals against multidrug resistant bacteria, in-vitro and in-vivo study | ||||
| Microbes and Infectious Diseases | ||||
| Article 36, Volume 5, Issue 2, May 2024, Page 781-798 PDF (1.65 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.261910.1761 | ||||
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| Authors | ||||
Abeer S. Hassan1; Noha M.S. Abdelazim2; Ehsan A.B. Hassan3; Sherein G. Elgendy3; Niveen G. El-Gindy4; Hebatallah M. Hassan   3
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| 1Department of Pharmaceutics, Faculty of Pharmacy, South Valley University, Qena, Egypt | ||||
| 2Assiut General Hospital, Assiut, Egypt | ||||
| 3Department of medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt | ||||
| 4Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||||
| Abstract | ||||
| Background: Development of multidrug-resistant (MDR) organisms is becoming a critical clinical problem for the management of numerous bacterial infections. Investigating other antibacterial drugs is urgently needed that are nontoxic and effective by different mechanisms. Methods: This study was aimed to develop silver nanoparticles (AgNPs) utilizing a green synthetic approach involving highly solubilized curcumin in freeze dried nanocrystals (CrNC) as a natural flavonoid reducing agent. Results: The fabricated silver nanoparticles were typically found to be spherical in shape with particle size distribution in the range of 10–50 nm and zeta potential of -18.3 ± 2.69mV. UV-Vis spectroscopy presented a characteristic plasmon peak of silver at 410 nm. The minimal inhibitory concentrations (MICs) of the prepared AgNPs against Staphylococcus aureus ATCC® 43300™*, Escherichia coli ATCC® 8739™*, Pseudomonas aeruginosa ATCC® 27853™* and Klebsiella pneumoniae ATCC® 33495™* were 7.8, 1.9, 3.9 and 1.9 µg/ml, respectively. The in-vitro cytotoxicity on human lung fibroblast cells demonstrated that green synthesized silver nanoparticles were nontoxic at the MIC. The in-vivo study revealed that AgNPs loaded hydrogel presented an improved antibacterial efficacy and wound healing effect, with normal skin appearance when compared with silver sulfadiazine cream (Dermazin®). Conclusion: The present investigation suggests that green synthesized silver nanoparticles are going to be a promising therapeutic nontoxic antibacterial agent against MDR bacteria with improved wound healing efficacy. | ||||
| Keywords | ||||
| multidrug resistant organism; silver nanoparticles; green synthesis; antibacterial; wound healing | ||||
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