Genetic Behavior for Reverse Thermo-Sensitive Genic Male Sterility (RTGMS) through Segregating Generations of some Rice Genotypes in Egypt | ||||
| Journal of Sustainable Agricultural Sciences | ||||
| Article 1, Volume 43, Issue 1, March 2017, Page 1-8 PDF (628.58 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jsas.2017.3487 | ||||
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| Abstract | ||||
| FIFTY populations selected from F2 for two crosses i.e. cross I (M.J. 5460 S × Sakha 106) and cross II (M.J. 5460 S × GZ7768) were planted as F3 generation in 2013 season and twenty populations were selected from F3 and planted as F4 generation in 2014 season. The selected populations were studied for reverse thermo-sensitive genic male sterility (rTGMS) trait, some growth and yield traits were measured. The F4 populations means were lower than the means of F3 populations only in the days to heading trait while, F4 means were higher than F3 means in the other studied traits. These data indicated the improvement for most studied traits by selection. High heritability was estimated in broad sense for all growth and yield traits in both crosses as F3 and F 4generations. Heritability in narrow sense ranged between 35.79 % for number of tillers trait in cross I as F3 generation to 74.95 % for days to heading trait in cross I as F3 generation. The highest genetic advance from selection as percentage of mean (Δg %) was 63.72 for yield per plant trait in cross II in F4 generation. Molecular studies indicated that, the RM222 as specific SSR primer revealed the presence of one specific band with molecular size of (200 bp) with M.J 5460S line as reverse thermo-sensitive genic male sterile line which indicated that, this band may be related to reverse thermo-sensitive genic male sterility gene. It could be concluded that RM222 as specific SSR primer could be used to identify the rtms1 gene in reverse thermo- sensitive genic male sterile lines, which could be used in japonica hybrid rice seed production u nder Egyptian conditions. | ||||
| Keywords | ||||
| rice; genotypes; sterility; genetic behavior | ||||
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