Egyptian Propolis Extract Attenuates Hepatotoxicity Induced by Doxorubicin via Increasing Antioxidant Defense and Decreasing Inflammatory and Apoptotic Markers: Targeting Nrf2 and Bcl-2 | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Volume 44, Issue 2, April 2024, Page 69-82 PDF (1.06 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2023.247742.1158 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ahmed Almeldin1; Reham lotfy Younis1; Rowida Raafat Ibrahim2; Shaimaa Motawea3; Mai Mwafy4; Haidy Abdelaziz Khattab   1
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| 1Physiology department ,Faculty of Medicine ,Tanta University , Tanta, Egypt | ||||
| 2Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt | ||||
| 3Anatomy Department, Faculty of Medicine, Tanta University, Tanta, Egypt | ||||
| 4Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Tanta, Egypt | ||||
| Abstract | ||||
| Doxorubicin (DOX) is a chemotherapy medication that is used to treat different types of cancers. Propolis is commonly used as a hepatoprotective agent against oxidative stress. Therefore, the present study was designed to investigate the possible protective role of the Egyptian propolis extract (EPE) against DOX-induced hepatic toxicity in rats. The study was carried out on forty male adult albino rats divided into four groups (control group): received normal saline by oral gavage daily for 28, (EPE group): received EPE (200 mg /kg) daily by oral gavage for 28 days. (DOX group): rats were injected once with DOX (20 mg/kg) intraperitoneally on the 24th day (EPE treated DOX group): received EPE (200 mg /kg) daily by oral gavage for 28 days and injected with DOX 20 mg/kg intraperitoneally on the 24th day. Our results revealed that liver enzymes, MDA, TNFα, interleukin -1β (IL-1β) and IL-6 and caspase-3 were significantly increased in DOX group compared with control, while EPE treated DOX group showed significant decrease. Catalase and superoxide dismutase were significantly decreased in DOX group compared with control while EPE treated DOX group showed significant increase. Moreover, gene expression of TNF α, nuclear factor erythroid 2–related factor 2 (NRF-2), heme oxygenase -1 (HO-1) have been elevated significantly in DOX group when compared with control and their mRNA levels have been downregulated significantly by EPE treatment while EPE treatment has upregulated gene expression of BCL-2. Conclusion: our results raised the idea that EPE protecting the liver from DOX-related oxidative and apoptotic effects. | ||||
| Keywords | ||||
| Hepatotoxicity; EPE; Nrf2; Bcl-2 | ||||
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