Effect of Xanthenone Versus Irisin in Alleviating Renal Ischemic Reperfusion Injury through Modifying the PI3K/AKT/eNOS and TLR-4/NFkB Pathways
Kolieb, E., El-shaer, R., Maher, S., Ali, D., Hammada, A., Awad, M. (2024). Effect of Xanthenone Versus Irisin in Alleviating Renal Ischemic Reperfusion Injury through Modifying the PI3K/AKT/eNOS and TLR-4/NFkB Pathways. EKB Journal Management System, 44(2), 83-105. doi: 10.21608/besps.2024.256207.1160
Eman Kolieb; Rehab Ahmed Ahmed El-shaer; Shymaa A. Maher; Dina A. Ali; Amal M. A. Hammada; Marwa Mahmoud Awad. "Effect of Xanthenone Versus Irisin in Alleviating Renal Ischemic Reperfusion Injury through Modifying the PI3K/AKT/eNOS and TLR-4/NFkB Pathways". EKB Journal Management System, 44, 2, 2024, 83-105. doi: 10.21608/besps.2024.256207.1160
Kolieb, E., El-shaer, R., Maher, S., Ali, D., Hammada, A., Awad, M. (2024). 'Effect of Xanthenone Versus Irisin in Alleviating Renal Ischemic Reperfusion Injury through Modifying the PI3K/AKT/eNOS and TLR-4/NFkB Pathways', EKB Journal Management System, 44(2), pp. 83-105. doi: 10.21608/besps.2024.256207.1160
Kolieb, E., El-shaer, R., Maher, S., Ali, D., Hammada, A., Awad, M. Effect of Xanthenone Versus Irisin in Alleviating Renal Ischemic Reperfusion Injury through Modifying the PI3K/AKT/eNOS and TLR-4/NFkB Pathways. EKB Journal Management System, 2024; 44(2): 83-105. doi: 10.21608/besps.2024.256207.1160

Effect of Xanthenone Versus Irisin in Alleviating Renal Ischemic Reperfusion Injury through Modifying the PI3K/AKT/eNOS and TLR-4/NFkB Pathways

Bulletin of Egyptian Society for Physiological Sciences
Volume 44, Issue 2, April 2024, Page 83-105  XML PDF (2.17 MB)
Document Type: Original Article
DOI: 10.21608/besps.2024.256207.1160
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Authors
Eman Kolieborcid 1; Rehab Ahmed Ahmed El-shaer email orcid 2; Shymaa A. Maherorcid 3; Dina A. Aliorcid 4; Amal M. A. Hammadaorcid 5; Marwa Mahmoud Awadorcid 2
1Physiology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
2Physiology Department, Faculty of Medicine, Tanta University, Tanta 31511, Egypt
3Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
4Clinical Pharmacology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
5Anatomy Department, Faculty of Medicine, Tanta University, Tanta 31511, Egypt;
Abstract
Ischemic reperfusion injury (IRI) in the kidney is a common cause of acute-kidney-injury. Our aim is to compare the effects of Xanthenone /Irisin on alleviating inflammation, cell apoptosis and oxidative stress injury in kidney IRI via modulating the PI3K/Akt/eNOS and TLR4-NFkB pathways. 32 male rats were equally divided into groups: Control, IR, IR treated with (i.v) Xanthenone (10mg/kg), IR treated with (i.p) Irisin (100 µg/kg). Renal functions, blood pressure, renal tissue ACE2, Ang 1-7, inflammatory, oxidative stress and apoptotic markers were evaluated. Also, PI3K/AKT/eNOS and TLR-4 relative mRNA levels in renal tissue were assessed. Also, the protein concentration of pAKT, eNOS, histomorphological, and immunohistochemical analysis were done. In IR group, renal function tests, blood pressure, and apoptotic parameters were elevated. ACE2 and Ang (1-7) tissue levels, relative gene expression of PI3K/AKT/eNOS and TLR-4, the protein concentration of pAKT and eNOS, histomorphological and immunohistochemical analysis of NFkB in renal tissue were all distorted in IR group. However, Xanthenone and Irisin treatments improved renal function, inflammation, and oxidative stress through PI3K/AKT/eNOS and TLR-4/ NFĸB pathway. Moreover, Xanthenone acts through ACE2/Ang (1-7) pathway, while Irisin acts mainly through PI3K/AKT/eNOS and TLR4/NFkB pathway. This may increase the potential for combined Xanthenone and Irisin therapy during conditions of AKI.
Keywords
Renal Ischemic/reperfusion; Xanthenone; Irisin; ACE2; Ang II
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