Ultraviolet-Visible Spectroscopy And Chemometrics Analysis Of Clover, Citrus And Sugar Feeding Honey.

Abd El Dayem, Mohamed; Kamel, Ahmed Ali; Marzouk, Weal Mahmoud; Hashish, Mohamed El_Sayied

doi:10.21608/ejchem.2024.215694.8746

	Ultraviolet-Visible Spectroscopy And Chemometrics Analysis Of Clover, Citrus And Sugar Feeding Honey.
Egyptian Journal of Chemistry
Volume 67, Issue 10, October 2024, Page 11-20 PDF (1.22 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.215694.8746
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Authors
Mohamed Abd El Dayem ¹; Ahmed Ali Kamel²; Weal Mahmoud Marzouk²; Mohamed El_Sayied Hashish³
¹Beekeeping Research Department, Plant Protection Res. Institute (PPRI), Agric. Res. Center, Giza, Egypt.
²Plant Protection Research Institute, Agricultural Research Centre, Nadi El-Said Street- Dokki, Giza, Egypt.
³Plant Protection Research Institute, Agricultural Research Centre, Nadi El-Said Street- Dokki, Giza, Egypt.
Abstract
Abstract This work evaluate fast and accurate analytical method model developed for the authentication of honey adulteration by sugar-feeding syrup using UV-VIS (Ultraviolet-visible) spectroscopy in absorbance mode together with chemometric techniques of principal component analysis (PCA) and canonical discrimination analysis, thirty-five honey samples from three types of honey (18 samples of clover honey,14 samples of citrus honey and 3 samples of sugar-feeding honey samples), were measured by a spectrophotometer with recorded wavelength range from 200 to 900 nm, in two different extract solution (water and ethanol), the method of PCA was used to lower the number of inputs (wavelengths), then, the principal components were used with canonical discrimination analysis on the following wavelength ranges individually: UV (200-400nm), VIS (400-900nm) and UV-VIS (200-900nm) for water and ethanolic extract honey samples. The results of the experiment clearly support for the UV-VIS spectral wavelengths with Chemometrics Analysis can be reduced at large spacing interval, which allows easing data analysis as well as developing a simpler and cheaper sensor for honey discrimination in practice. PCA and canonical discrimination were successfully able to present a preliminary clustering pattern to segregate the feeding sugar syrup honey samples from the clover and citrus honey sample in UV and UV-VIS region spectroscopy by water extract.
Keywords
UV-VIS spectroscopy; honey; adulteration; discrimination; PCA


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