Prevalence of multidrug-resistant Klebsiella pneumoniae in urinary tract infections: A retrospective observational study in eastern India

Sahoo, Swayamprabha; Mohanty, Jatindra Nath; Routray, Sweta Padma; Sarangi, Ashirbad; Nayak, Debasish Swapnesh Kumar; Shah, Sejal; Das, Jayashankar; Subudhi, Enketeswara; Swarnkar, Tripti

doi:10.21608/mid.2024.276619.1844

	Prevalence of multidrug-resistant Klebsiella pneumoniae in urinary tract infections: A retrospective observational study in eastern India
Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 05 April 2024
Document Type: Original Article
DOI: 10.21608/mid.2024.276619.1844
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Authors
Swayamprabha Sahoo ¹; Jatindra Nath Mohanty ²; Sweta Padma Routray ¹; Ashirbad Sarangi ¹; Debasish Swapnesh Kumar Nayak ³; Sejal Shah ⁴; Jayashankar Das⁵; Enketeswara Subudhi ⁶; Tripti Swarnkar ⁴
¹Centre for Biotechnology, Siksha ‘O’ Anusandhan Deemed to be University, Bhubaneswar, India
²Genetic Engineering and Genomic Laboratory, School of Applied Sciences, Centurion University of Technology and Management, Ramachandrapur, Jatni-752050, Odisha, India
³Department of Computer Science and Engineering, Siksha ‘O’ Anusandhan Deemed to be University, Bhubaneswar, India
⁴Department of Bioinformatics, Faculty of Technology, Marwadi University, Rajkot, India
⁵Valnizen Healthcare, Vile Parle, Mumbai, 40056, Maharastra, India
⁶Centre for Biotechnology, Siksha ‘O’ Anusandhan University, Khandagiri, Bhubaneswar, Odisha, 751003, India
Abstract
Background: Urinary tract infection (UTI) commonly affects people of different ages. It is important to explore the antibiotic susceptibility of the bacterial agents to improve the empirical antibacterial prescription to tackle the fast emerging of multi-drug resistant (MDR) bacteria. Klebsiella pneumoniae, a Gram-negative bacterium, is widely known for its hypervirulence, drug resistance and opportunistic nature, with significant implications for critically ill or immunocompromised patients. The study aim is to determine the prevalence of antibiotic resistance pattern among isolates from urinary tract infections (UTIs) and provide valuable insights for healthcare management. Methods: This is a retrospective observational study including 250 patients among 500 sample screened to have UTI at the largest hospital in eastern India from July 2020 to November 2022. All UTI patients underwent agar-based bacterium identification and antibiotic sensitivity testing using the disc diffusion method. Results: Klebsiella pneumoniae (122 cases) and Escherichia coli (74) were the first and second most frequent Gram-negative isolates, followed by Pseudomonas aeruginosa (45) and Acinetobacter baumannii (9). Notably, ten Klebsiella pneumoniae strains were resistant to five major antibiotic classes: β-lactam, aminoglycosides, fluoroquinolones, tetracyclines, and Polymyxin. Piperacillin/Tazobactam, Ceftazidime, Aztreonam, and Imipenem exhibited high resistance rates when tested against Klebsiella pneumoniae isolates. In contrast, Gentamicin, Levofloxacin, Minocycline, Fosfomycin, and Colistin demonstrated relatively lower effectiveness against Klebsiella pneumonia isolates. Conclusion: Monitoring of multidrug-resistant bacteria becomes critical for prudent antibiotic use, ultimately dropping the prevalence of hospital-acquired infections and contributing to the larger fight against antimicrobial resistance.
Keywords
Multidrug-resistant; Klebsiella pneumonia; Urinary tract infection (UTI); healthcare management; Gram-negative bacteria


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