Preventive impact of Soybean and black seed extracts against colon cancer progression through stimulation of apoptosis

Higazy, Radwa H.; Khalil, Wagdy K. B.; Othman, Othman E.; Youssef, Hend F.; Ali, Nagwa Hassan

doi:10.21608/ejchem.2024.269355.9312

	Preventive impact of Soybean and black seed extracts against colon cancer progression through stimulation of apoptosis
Egyptian Journal of Chemistry
Volume 67, Issue 10, October 2024, Page 327-340 PDF (927.66 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.269355.9312
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Authors
Radwa H. Higazy ¹; Wagdy K. B. Khalil ¹; Othman E. Othman ¹; Hend F. Youssef²; Nagwa Hassan Ali³
¹Cell Biology Department, Biotechnology research institute, National Research Centre, Giza, Egypt
²Tissue culture unit, pathology department, National cancer institute, Cairo University, Egypt
³Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
Colorectal cancer is considered one of the most occurring cancers leading to death. Apoptosis is defined as a programmed cell death controlled by different gene families via intrinsic and extrinsic pathways. Soybean and black seed have been found to possess anti-proliferative, anticancer, and anti-chemo-resistant properties. In this study, we assessed the preventive impact of soybean and black seed extracts different concentrations and their mixture against HT-29 colon cancer cell line through apoptosis using flow cytometry, DNA fragmentation assays and expression of six genes related to apoptosis. Flow cytometry analysis of the mixture of soybean and black seed extracts showed the highest total apoptosis (early and late apoptosis) and low necrosis percentages when compared to cisplatin and black seed extract at IC50. DNA fragmentation increased significantly among the groups treated with the mixture of the extracts with the highest fragmentation percentage. Expression analysis revealed a significant downregulation of P53, BIRC5, BCL2L1 and XIAP genes and a significant upregulation of Bax genes. Whereas FAS gene was expressed without any significant difference in all groups compared to control. The mixture demonstrated the most significant difference in each gene among the groups. As a result, mixture exhibited pro-apoptotic effect through different analyses.
Keywords
Soybean (Glycine max); black seed (Nigella sativa); HT-29 colon cancer; apoptosis; DNA fragmentation; apoptotic gene expression


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