Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes
Yassen, D., Abd El-Gawad, E., Mahrous, K., Abd El-Razik, K., Tantawy, A., Abbass, A. (2024). Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes. EKB Journal Management System, 46(1), 102-107. doi: 10.21608/bvmj.2024.267524.1783
Doaa Yassen; Eman Abd El-Gawad; Karima Mahrous; Khaled Abd El-Razik; Ahmed Tantawy; Amany Abbass. "Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes". EKB Journal Management System, 46, 1, 2024, 102-107. doi: 10.21608/bvmj.2024.267524.1783
Yassen, D., Abd El-Gawad, E., Mahrous, K., Abd El-Razik, K., Tantawy, A., Abbass, A. (2024). 'Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes', EKB Journal Management System, 46(1), pp. 102-107. doi: 10.21608/bvmj.2024.267524.1783
Yassen, D., Abd El-Gawad, E., Mahrous, K., Abd El-Razik, K., Tantawy, A., Abbass, A. Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes. EKB Journal Management System, 2024; 46(1): 102-107. doi: 10.21608/bvmj.2024.267524.1783

Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes

Benha Veterinary Medical Journal
Article 19, Volume 46, Issue 1, April 2024, Page 102-107  XML PDF (765.27 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2024.267524.1783
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Authors
Doaa Yassen1; Eman Abd El-Gawad email orcid 1; Karima Mahrous2; Khaled Abd El-Razik3; Ahmed Tantawy4; Amany Abbassorcid 1
1Aquatic Animal Medicine Department, Faculty of Veterinary Medicine, Benha University Egypt
2Cell Biology Department, Biotechnology Research Institute National Research Center (NRC), Egypt
3Animal Reproduction Department, Veterinary Research Institute, National Research Center (NRC), Egypt .
4Pathology Department, Faculty of Veterinary Medicine, Benha University, Egypt
Abstract
The present study aimed to molecularly diagnose microsporidia in Nile tilapia and African catfish at the Qalyubia Governorate in Egypt and describe the histopathological alterations that are related with them. Seventy-nine out of 400 (19.75%) Nile tilapia, and forty-eight out of 200 (24%) African catfish had white nodules in their liver and kidneys were collected from natural water resources. BLAST analysis showed that 18S rDNA of isolated Glugea anomala from Nile tilapia (acc. no. OR766288) and from African catfish (acc. no. OR766287) revealed 100% sequence similarity with Glugea anomala (AF044391, AB923879) and Glugea arabica (KT005391). The highest seasonal infection rates with Glugea anomala in Nile tilapia and African catfish were recorded in autumn (32%) and spring (36%) respectively. Histopathological examination revealed the presence of microsporidian spores in the cytoplasm of degenerated and necrotic hepatocytes and renal tubular epithelium. On conclusion, the study recorded Glugea anomala in naturally infected Nile tilapia and African catfish that negatively alter the tissue and hence fish health status.
Keywords
African Catfish; Glugea anomala; Histopathology; Nile tilapia; 18S ribosomal DNA
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