Prevalence and bacteriological investigation of Bacillus cereus isolated from meat and milk products in El-Gharbia governorate, Egypt. | ||||
| Benha Veterinary Medical Journal | ||||
| Article 23, Volume 46, Issue 1, April 2024, Page 125-129 PDF (369.57 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2024.260058.1770 | ||||
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| Authors | ||||
Shimaa Ibrahim Mohamed rashad Elhaw  1; Seham Nazeeh Homouda2; Ashraf Awad Abd El-tawab 1
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| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Egypt. | ||||
| 2Department of Food Hygiene, Animal Health Research institute (AHRI) Tanta Branch, Agricultural Research Center (ARC), Egypt. | ||||
| Abstract | ||||
| Bacillus cereus is a food-borne pathogen that can cause emesis, diarrhea and spoilage of different food products. For this, 125 random samples of meat products (luncheon and sausage) and milk products (ras or romy cheese, karish cheese and rice pudding), 25 of each, were taken from various shops and marketplaces in El-Gharbia governorate, Egypt to be examined for prevalence, isolation and identification of Bacillus cereus. Additionally, the significance of Bacillus cereus for public health was covered. The bacteriological examination of collected samples showed that, a total of 40/125 (32%) Bacillus cereus strains were obtained; mostly they were isolated from Ras cheese 14/25 (56%) followed by Luncheon 11/25 (44%), Rice pudding 8/25 (32%) then from Sausage 7/25 (28%) and there is no isolated Bacillus cereus from Karish cheese 0/25 (0%). Polymerase chain reaction for five random isolated studied strains showed that groEL (diagnostic and marker gene of Bacillus cereus) gene was detected in all five studied strains. The anti-microbial sensitivity test for 20 Bacillus cereus strains revealed that they were completely sensitive to ciprofloxacin, norfloxacin and levofloxacin (100%) followed by gentamycin (80%), doxycycline (60%), chloromphenicol (50%), erythromycin (30%) then clindamycin (20%), whereas they were completely resistant to ampicillin, cefipime, aztreonam and amoxicillin-clavulanic acid. | ||||
| Keywords | ||||
| Bacillus cereus; meat and milk product; antimicrobial sensitivity; PCR; groEL | ||||
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