Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.
Dowidar, M., Homouda, S., Abd El Tawab, A. (2024). Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.. EKB Journal Management System, 46(1), 119-124. doi: 10.21608/bvmj.2024.262505.1793
Maryam Said Fouad Dowidar; Seham Nazeeh Homouda; Ashraf Awad Abd El Tawab. "Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.". EKB Journal Management System, 46, 1, 2024, 119-124. doi: 10.21608/bvmj.2024.262505.1793
Dowidar, M., Homouda, S., Abd El Tawab, A. (2024). 'Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.', EKB Journal Management System, 46(1), pp. 119-124. doi: 10.21608/bvmj.2024.262505.1793
Dowidar, M., Homouda, S., Abd El Tawab, A. Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.. EKB Journal Management System, 2024; 46(1): 119-124. doi: 10.21608/bvmj.2024.262505.1793

Antibiogram Pattern and Molecular Characterization of Multiple drug resistant Salmonella Isolated from Different Food Products in Egypt.

Benha Veterinary Medical Journal
Article 22, Volume 46, Issue 1, April 2024, Page 119-124  XML PDF (444.26 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2024.262505.1793
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Authors
Maryam Said Fouad Dowidar email orcid 1; Seham Nazeeh Homouda2; Ashraf Awad Abd El Tawaborcid 3
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor
2Department of Food Hygiene, Animal Health Research Institute, Tanta Branch, Agricultural research center (ARC)
3Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
Abstract
Non-typhoidal Salmonellosis is a major foodborne infection. The objectives of our study were to investigate the prevalence rates of multiple drug resistant (MDR ) Salmonella spp in various food products, A total number of 100 food samples including 20 samples for each of kariesh cheese ,raw milk , chicken fillet and minced meat and 10 samples for each of chicken liver and raw meat were collected from February to July of 2022 within different markets in El-Gharbia,Egypt. All samples were subjected for isolation and identification of Multiple drug resistant Salmonella by cultivation on xylose lysine deoxycholate (XLD) agar , biochemical test ,serological test , antibiograms and the Polymerase Chain Reaction (PCR) screen of virulence gene (invA ) ,class 1 integron and resistant genes (blaTEM ,qnrS and aadA1). The incidence of Salmonella was 2(2%) and based on molecular serotyping were identified as S. Kentucky and S.Typhimurium . All Salmonella isolates (100%) showed MDR . Variable antibiotic resistance patterns were detected from 100% for Ampicillin ,norfloxacin and gentamicin to 50% for Doxycycline and ciprofloxacin. Based on PCR analysis, invA, class (1) integron ,blaTEM, qnrs and aadA1 were detected in (100% ) of all identified Salmonella isolates .
Keywords
Keywords : blaTEM; class 1 integron; Multiple drug resistant (MDR); PCR and qnrS
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