Gene sequences of TNF and INFα Cytokines in Cryptosporidium Co-infection with H-pylori of irritable bowel syndrome

Nasralla, Basheer Abd.; Mohammed, Nazar Sh.; Muhsen, Salwa S.

doi:10.21608/jbaar.2024.350195

	Gene sequences of TNF and INFα Cytokines in Cryptosporidium Co-infection with H-pylori of irritable bowel syndrome
Journal of Bioscience and Applied Research
Articles in Press, Corrected Proof, Available Online from 15 April 2024
Document Type: Original Article
DOI: 10.21608/jbaar.2024.350195
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Authors
Basheer Abd. Nasralla¹; Nazar Sh. Mohammed ²; Salwa S. Muhsen¹
¹Medical Technical Institute, Middle Technical University (MTU), Iraq
²Collage of Health and Medical Techniques, Meddle Technical University (MTU), Iraq
Abstract
In this study, 100 whole blood samples were collected from patients having Cryptosporidiosis and co-infected with H pylori bacteria who attended Baghdad Teaching Hospital during the period from June 2022 to April 2023. The results showed that the Mean±S.E of anti-Cryptosporidium IgM antibodies in the patient group was (14.614±0.901) when compared with the control group (0.081±0.022), with a highly significant difference p<0.0001. Also, the Mean±S.E of anti-Cryptosporidium IgG antibodies was (24.213±0.839) in the patient group when compared with the control group (4.775±0.249), with a highly significant difference p<0.0001. the Mean±S.E of anti-H pylori IgM antibodies in the patient group was (13.923±1.005) when compared with the control group (0.200±0.032), with a highly significant difference p<0.0001. The results also demonstrated that the Mean±S.E of anti-H pylori IgG in the patient group was (28.460±0.847) when compared with the control group (15.835±0.438), with a highly significant difference p<0.0001. The result showed that Mean±S.E of INFα in the patient group was (14.588±0.849) when compared with the control group (4.655±0.271), with a highly significant difference p<0.0001. In acute infection with H pylori, the Mean±S.E of H pylori patients was (13.498±0.965^a), p<0.0001. Data in our study also showed a mutation occurred with TNF gene ID 90865 in SNPs, rs7044343. The variation of wild TT was changed to CC, CC, TC, TC, CC, TC, CC, TC, TT, TC in comparison with the control group. A mutation occurred with INFα gene ID 90865 in SNPs, rs7044343. The variation of wild TT was changed to CC, CC, TC, TC, CC, TC, CC, TC, TT, TT in comparison with the control group.
Keywords
Gene sequences; TNF; INFα; Cryptosporidium; Co-infection; H-pylori


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