ANTIOXIDANT CAPACITY OF CYSTEAMINE ON BUFFALO GRANULOSA CELL PERFORMANCE UNDER OXIDATIVE STRESS CONDITIONS | ||||
| Egyptian Journal of Animal Production | ||||
| Article 4, Volume 61, Issue 1, January 2024, Page 31-37 PDF (738.11 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejap.2024.250268.1065 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Marwa Faheem   ; Dalia Ahmed; Sherif Dessouki
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| Animal Production Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt | ||||
| Abstract | ||||
| The impact of antioxidant supplementation of cysteamine during in vitro culture of buffalo granulosa cells (GCs) was evaluated in relation to their viability and enzymatic activities under hydrogen peroxide (H2O2)-induced oxidative stress. Mural GCs were harvested, cultured for 24 h of confluence at 37.5ºC and then exposed to 500 µM H2O2 for 30 min followed by cysteamine supplementation (0, 100, 200, 300 and 400 µM) for further 24 h of culture and their counterpart-control groups were supplemented with cysteamine without H2O2 pre-treatment. Granulosa cell viability was assessed using neutral red assay, while the culture conditioned media were subjected to enzymatic analyses. Our results revealed that GCs viability rate was decreased by H2O2 pre-treatment; being 63.0±14.0, 61.8±14.1, and 61.8±10.3 % for 100, 300, and 400 µM of cysteamine, respectively, except increasing viability for 200µM cysteamine (80.0±10.0 %) as compared to control (65.2±4.0). However, the viability was insignificantly enhanced by cysteamine levels under normal physiological conditions. For enzymatic analyses, comparable GSH-Px activity was monitored for all groups of the study. Catalase activity was consistent in 100 and 200 µM of cysteamine under H2O2 or normal physiological condition. While a significant reduction was observed in 300 µM of cysteamine under H2O2 pre-treatment, there were no significant differences between all groups of the study for malondialdehyde (MDA) levels. In conclusion, H2O2 negatively affected GCs viability; however, 200 µM of cysteamine ameliorated cells viability under oxidative stressful condition. The antioxidant enzymes GSH-Px/CAT acted in synergistic dynamics against oxidative stress. | ||||
| Keywords | ||||
| Granulosa cells; Oxidative stress; Cysteamine; Hydrogen peroxide; GSH-Px | ||||
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