Molecular Detection of Monocyte Chemotactic Protein-1 gene Polymorphism in Patients with Spontaneous Bacterial Peritonitis | ||||
Bulletin of Egyptian Society for Physiological Sciences | ||||
Article 7, Volume 33, Issue 1, June 2013, Page 85-92 PDF (289.92 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/besps.2013.35178 | ||||
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Authors | ||||
Maysa Salama* 1; Dina Sabry1; Rasha Ahmed2; Fatma Taha1; Miriam Wadie1 | ||||
1Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University | ||||
2Department of Tropical Medicine, Faculty of Medicine, Cairo University | ||||
Abstract | ||||
Backgrounds and Aim: Patients with cirrhosis and ascites show a higher susceptibility to bacterial infections,Monocyte Chemotactic Protein-1(MCP-1) secretion is upregulated during chronic hepatitis and correlates with the severity of hepatic inflammation. We thought to confirm the association of the functional MCP-1 promoter polymorphism (A-2518G) with spontaneous bacterial peritonitis (SBP). Methods: 50 patients with post-hepatitis C virus liver cirrhosis and ascites categorized into two groups, group I: 25 patients with SBP, group II: 25 patients free from SBP, in addition a group of 20 healthy volunteers were included. We assessed the MCP-1 gene polymorphism in blood. Results: Significant MCP-1 gene polymorphism was detected in group I & II (p-value=0.001 & 0.02 respectively), group I was significantly associated with AG genotype, group II with GG genotype when compared to healthy volunteers (p-value=0.002 & 0.001 respectively), and G allele was significantly higher in both groups (I & II) (p-value 0.005 & 0.001 respectively). Conclusion: MCP-1 GG genotype and G allele in HCV infected patients may be associated with more advanced stage of the disease. AG genotype may increase the susceptibility to spontaneous bacterial peritonitis. | ||||
Keywords | ||||
genotype; allele; ascites; Liver cirrhosis; Gene expression | ||||
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