Influence of MiR-126-3p and MiR-146a-5p Dysregulation on The Risk and Clinicopathological Features of Papillary Thyroid Cancer in Patients with Thyroid Nodules

Rashad, Nearmeen; Abdel Aziz, Eman; abdelsamad, MONA; Mohey, Nesreen M; Abd El-Fatah, Azza Hassan; mousa, mayada

doi:10.21608/zumj.2024.282351.3328

	Influence of MiR-126-3p and MiR-146a-5p Dysregulation on The Risk and Clinicopathological Features of Papillary Thyroid Cancer in Patients with Thyroid Nodules
Zagazig University Medical Journal
Articles in Press, Accepted Manuscript, Available Online from 27 April 2024
Document Type: Original Article
DOI: 10.21608/zumj.2024.282351.3328
View on SCiNiTO
Authors
Nearmeen Rashad ¹; Eman Abdel Aziz²; MONA abdelsamad³; Nesreen M Mohey ⁴; Azza Hassan Abd El-Fatah⁵; mayada mousa⁶
¹ZAagazig university
²clinical pathology department, faculty of medicine, Zagazig University
³Medical analysis laboratory, Student Hospital, Zagazig University. Egypt.
⁴Radiodiagnosis department faculty of medicine Zagazig University
⁵Lecturer of Internal Medicine, Faculty of Medicine, Zagazig University
⁶zagazig university
Abstract
Background Papillary thyroid cancer (PTC) is the most frequent endocrine cancer. Aberrant expression of miRNAs can increase the risk of thyroid cancer. We aimed to investigate the miR-126-3p and miR-146a-5p plasma levels in patients with thyroid nodules and to identify their associations with clinicopathological characteristics, and progression of PTC. Methods: We enrolled 40 healthy control and forty patients with thyroid nodules; benign thyroid nodules (BTN), (n=30), and PTC(n=10). We analyzed miR-126-3p and miR-146a-5p levels by using real-time PCR. Results: miR-126-3p expression level was downregulated in PTC patients (0.40±0.11) compared with BTN (0.58±0.12) and healthy subjects (0.87±0.31), P<0.001*Regarding miR-146a-5p, its level was upregulated in PTC patients (4.59±1.61 ) compared with BTN (3.77±0.96) and healthy subjects (0.99±0.41), Additionally, miR-126-3p and miR-146b-5p levels were significantly associated with tumor progression, lymph node metastasis, TNM staging, and thyroid function tests. The diagnostic power of miR-126-3p in the prediction of PTC, the AUC was 0.860 with a sensitivity of 60 % and specificity of 87.3% at cutoff values =0.49. miR-146a-5p level in the prediction of PTC, the AUC was 0.742 with a sensitivity of 80 % and specificity of 74.3% at cutoff values =3.4.
Keywords
miR-146b-5p; MiR-126-3p; papillary thyroid cancer; benign thyroid nodules; real-time PCR


Statistics Article View: 25