Protective Effect Of Diosmin-Hesperidine Combination On Gamma Radiation-Induced Apoptosis In Liver Of Albino Rats. Molecular And Immunohistchemistry Studies | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 67, Issue 11, November 2024, Page 571-584 PDF (1.2 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.267548.9281 | ||||
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| Authors | ||||
Hazem K.A. Sarhan  1; Ahmed M.A. Saleh2; Olfat A.E. Hammam3; Aly H. Atta4; Islam M. El-Nehrery4
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| 1Medical and Radiation Research Department, Research Sector, Nuclear Material Authority, Cairo, Egypt | ||||
| 2Head of Giza scientific office, Egyphar for Pharmaceutical Idustry, El-Obbore, Cairo, Egypt | ||||
| 3Pathology Department, Thiodor Bellharz Inistitute for Research, Ministry of Scientific Research, Cairo, Egypt. | ||||
| 4Chemistry Department, Faculty of Science, Suiez University, Suiez, Egypt | ||||
| Abstract | ||||
| This study used radiation-induced damage in Wistar albino rats as a proxy for assessing the radio and antioxidant protective efficacy of diosmin-hesperidin, a natural plant citrus flavone of hesperidin derivative. Before being exposed to a cumulative dose (10Gy) of gamma radiation in the form of fractionated dose (2 Gy for 5 times every other day), rats were given diosmin-hesperidin dose (200 and 100 mg/kg body wt. correspondingly) orally for a month (every other day). The purpose of this study is to assess the diosmin-hesperidin combination's radioprotective and antioxidant efficacy. Apoptosis was evaluated using several methods, including real-time polymerase chain reaction (RT-PCR) analysis, biochemical analysis, histopathological changes, the comet assay, and caspase-3 activity. As shown by the findings, pretreatment with diosmin+hesperidine counteracted the detrimental effects of radiation on antioxidant indices (SOD, GPx, and GSH), lipid peroxidation, DNA damage, and apoptosis. Lipid peroxidation, DNA damage, and tissue damage were all mitigated by a diosmin+hesperidine dose (200 and 100 mg/kg body wt., respectively) that brought antioxidant status back to near normal. Histopathological analyses corroborated these findings, demonstrating that the livers of albino rats were protected from gamma-irradiation-induced damage when diosmin+hesperidine was given prior to the radiation exposure. So, the evidence suggests that diosmin+hesperidine can protect rats from radiation-induced harm. In addition, there were no signs of metabolic changes or DNA damage in the diosmin+hesperidine pretreatment group. | ||||
| Keywords | ||||
| Radioprotection; reactive oxygen species; Rt-PCR; DNA damage; and apoptosis | ||||
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