Morphological and Pcr Differentiation between some Phages from Bacillus thuringiensis Strain C18 | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 9, Issue 8, August 2018, Page 169-173 PDF (1.32 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2018.35245 | ||||
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| Authors | ||||
| Y. Osman; A. El-Morsi; Amany Atiya | ||||
| Botany Department, Faculty of Science, Mansoura University, Egypt | ||||
| Abstract | ||||
| Bacillus thuringiensis (Bt)-based pesticides hold the biggest share of the biopesticides market. This industry suffers huge economic losses due to the activation of their lysogenic phages during fermentation process. The locally isolated wide-spectrum BtaC18 is currently used for commercial production and found to harbors many of these integrated prophages. Many phages were isolated from a laboratory-grown BtaC18; from amongst them three belonged to the order Caudovirales were characterized. Traditional and morphological examination found that one of the three viruses has tail: ACTP-01 and two are non-tailed phages: ACTP-02 and ACTP-03. The ACTP-01 phage showed an isometric head (92.6 nm) with long non-contractile, flexible tail (321.33 x 12.5 nm). While, the ACTP-02 phage has a hexagonal head (50.82 nm) and the ACTP-03 phage possesses an icosahedral head (61.3 nm). Data suggested that ACTP-01 phage belongs to Siphoviridae family,while ACTP-02 and ACTP-03 belonged to the Tectiviridae family. Random Amplified Polymorphic DNA polymerase chain reaction (RAPD-PCR) showed a strong differentiating power between the isolated phages. In conclusion, the numerous phages hosted by this bacterium complicate the derivatization of phage resistant BtaC18 strain. | ||||
| Keywords | ||||
| Bacteriophages; Bacillus thuringiensis; Bacillus thuringiensis subsp. aegypti strain C18; RAPD-PCR | ||||
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