Effects of Immobilization Stress and Adrenomodullin on Interleukin-10 Levels in Some Rat Tissues | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 12, Volume 33, Issue 2, December 2013, Page 161-171 PDF (381.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2013.35257 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A Gadalla* 1; A Al Gendy2; A Hashim3; M Amer4; O Sabry5 | ||||
| 1Department of Physiology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt. | ||||
| 2Department of Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt. | ||||
| 3Department of clinical Pathology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt | ||||
| 4Department of clinical Pathology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt, | ||||
| 5Department of haematology , Theodor Bilharze,Research institute, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Immobilization stress known to stimulate sympathetic activity, as well as the hypothalamic–pituitary–adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secretion of proinflammatory cytokines. Interleukin (IL)-10 is a potent activator of the HPA and appears to play a pathogenic role in conditions related to stress Objective: The aim of this study was to evaluate and validate the effects of AdM administration and immobilization stress treatment on IL-10 levels in rat liver, lung, brain and heart tissues. Materials and Methods: The study was carried out on twenty-four male albino rats (8 months old, 190–240 g). The animals were divided into 4 groups of 6 rats each group. Group A : control group. Group B : AdM-treated group , rats received intraperitonealy (i.p.) injection of AdM (2000 μg/g body weight) once daily for 1 week. Group C : immobilized stress group ,(rats were immobilized by keeping them into transparent plastic jars with 5 holes for 4hrs per day for 1 week).Group D: immobilized stress +AdM group. Rats were immobilized by keeping them into transparent plastic jars with 5 holes for 4hrs a day for 1 week and were given AdM i.p at a single dose of 2000 μg/g body weight for a week. At the end of the experiment, the concentration of IL-10 was determined using an enzyme-linked immunosorbent assay (ELISA) kit. Results: The results of the present study showed that IL-10 levels increased in all tissues in immobilized stress group when compared to control , also IL-10 levels were increased in AdM treatment group in all tissues when compared to control . IL-10 levels were decreased in the immobilization stress + AdM treatment group in all tissues when compared to the stress group, increased IL-10 levels in brain and lung tissue but decreased in heart and liver tissues in the immobilization stress + AdM treatment group when compared to the AdM treatment group. Conclusion: The results suggest that immobilization stress may induce increase of rat proinflammatory cytokine IL-10 and AdM may play a regulatory or protective role for immobilization stress. | ||||
| Keywords | ||||
| Immobilization stress; Adrenomedullin; Interleukin 10 | ||||
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