Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis
Ajdig, M., Rached, B., Mbarki, A., Chouati, T., Talbi, C., El Fahime, E., Melloul, M. (2024). Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis. EKB Journal Management System, 8(3), 2414-2434. doi: 10.21608/nrmj.2024.280135.1508
Mohammed Ajdig; Bahia Rached; Ahlam Mbarki; Taha Chouati; Chouhra Talbi; Elmostafa El Fahime; Marouane Melloul. "Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis". EKB Journal Management System, 8, 3, 2024, 2414-2434. doi: 10.21608/nrmj.2024.280135.1508
Ajdig, M., Rached, B., Mbarki, A., Chouati, T., Talbi, C., El Fahime, E., Melloul, M. (2024). 'Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis', EKB Journal Management System, 8(3), pp. 2414-2434. doi: 10.21608/nrmj.2024.280135.1508
Ajdig, M., Rached, B., Mbarki, A., Chouati, T., Talbi, C., El Fahime, E., Melloul, M. Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis. EKB Journal Management System, 2024; 8(3): 2414-2434. doi: 10.21608/nrmj.2024.280135.1508

Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis

Novel Research in Microbiology Journal
Volume 8, Issue 3, May and June 2024, Page 2414-2434  XML PDF (728.61 K)
Document Type: Original Article
DOI: 10.21608/nrmj.2024.280135.1508
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Authors
Mohammed Ajdigorcid 1; Bahia Rachedorcid 2; Ahlam Mbarkiorcid 3; Taha Chouatiorcid 4; Chouhra Talbiorcid 1; Elmostafa El Fahimeorcid 4; Marouane Melloul email orcid 3
1Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University, Rabat, Morocco
2Genopath Research Center, ERNN, Faculty of Medicine and Pharmacy, University Mohammed V of Rabat, Morocco
3Functional Genomic Platform/National Centre for Scientific and Technical Research (CNRST), Rabat, Morocco
4Laboratory of Chemistry-Physics and Biotechnology of Biomolecules and Materials, Faculty of Science and Technology, Mohammedia, Hassan II University, Casablanca, Morocco
Abstract
Bacillus paralicheniformis is a new identified species, which was distinguished from Bacillus licheniformis in 2015 through extensive phylogenomic and phylogenetic analyses. In this context, this study aimed to achieve a clear identification of the active plant-growth promoting rhizobacteria (PGPR) B. paralicheniformis isolates among the closely related B. licheniformis through molecular typing, helping for the development of clearly-identified PGPR isolates to be used as biofertilizers. A total of 15 rhizobacteria were isolated from the olive rhizosphere soils. These bacterial isolates exhibited various proprieties in terms of abiotic stress tolerance, biofilm formation under stress conditions, and enzyme activities (i.e., lipases, cellulases, and proteases). In addition, several PGP traits such as phosphate solubilization and the production of siderophores and indol-3 acetic acid were also observed. Molecular identification through 16S rRNA sequencing initially identified all the isolates as Bacillus spp. The multi-locus sequence typing (MLST) scheme, using six housekeeping genes (adk, ccpA, recF, rpoB, sucC, and spo0A) had unveiled distinct allelic profiles resulting in 13 unique sequence types (ST). Notably, a comprehensive analysis indicated no exact allele matches existed between the examined isolates and those documented in the PubMLST database. Among the six housekeeping genes, we noticed that rpoB gene (RNA polymerase, subunit beta) had multiple polymorphic sites that were bordered by conserved sequences. Furthermore, the phylogenetic analysis based on rpoB had identified clusters indicating distinct phylogenetic relationships between B. paralicheniformis and B. licheniformis, and successfully differentiated the two species from a pool of 90 strains. The rpoB partial sequence proved to be effective for accurate species discrimination.
Keywords
Bacillus paralicheniformis; Bacillus licheniformis; rpoB gene; PGPR; MLST
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