Evaluation of the in-vitro anti-inflammatory and hemolytic activity of aqueous extracts of Silene Vulgaris (Moench) Garcke collected from Algeria.
El hadj berrezig, A., Khaldi, A., Mehdi, K., Yazid, A. (2024). Evaluation of the in-vitro anti-inflammatory and hemolytic activity of aqueous extracts of Silene Vulgaris (Moench) Garcke collected from Algeria.. EKB Journal Management System, 10(2), 193-200. doi: 10.21608/jbaar.2024.282805.1044
Abdelkader El hadj berrezig; Achraf Khaldi; Kheira Mehdi; Amar Yazid. "Evaluation of the in-vitro anti-inflammatory and hemolytic activity of aqueous extracts of Silene Vulgaris (Moench) Garcke collected from Algeria.". EKB Journal Management System, 10, 2, 2024, 193-200. doi: 10.21608/jbaar.2024.282805.1044
El hadj berrezig, A., Khaldi, A., Mehdi, K., Yazid, A. (2024). 'Evaluation of the in-vitro anti-inflammatory and hemolytic activity of aqueous extracts of Silene Vulgaris (Moench) Garcke collected from Algeria.', EKB Journal Management System, 10(2), pp. 193-200. doi: 10.21608/jbaar.2024.282805.1044
El hadj berrezig, A., Khaldi, A., Mehdi, K., Yazid, A. Evaluation of the in-vitro anti-inflammatory and hemolytic activity of aqueous extracts of Silene Vulgaris (Moench) Garcke collected from Algeria.. EKB Journal Management System, 2024; 10(2): 193-200. doi: 10.21608/jbaar.2024.282805.1044

Evaluation of the in-vitro anti-inflammatory and hemolytic activity of aqueous extracts of Silene Vulgaris (Moench) Garcke collected from Algeria.

Journal of Bioscience and Applied Research
Volume 10, Issue 2, June 2024, Page 193-200  XML PDF (591.76 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2024.282805.1044
View on SCiNiTO View on SCiNiTO
Authors
Abdelkader El hadj berrezig email orcid 1; Achraf Khaldiorcid 2; Kheira Mehdiorcid 2; Amar Yazidorcid 3
1Biology departement, nature science and life faculty, Mohamed Tahri university , Bechar , Algeria
2Department of Biology, Faculty of Natural Science and Life ,Tahri Mohammed University,Bechar , Algeria
3Department of Biology, Faculty of Natural science and life, University Ibn Khaldoun, Tiaret , Algeria
Abstract
Silene vulgaris (Moench) Garcke is a popular edible plant that is consumed by a significant population in many countries. The present research focused on determining the in-vitro anti-inflammatory ability and hemolytic activity of aqueous extracts derived from the soil and aerial parts of S. vulgaris. The study involved many plant parts, including roots, leaves, stems, and flowers. The extraction method adopted consisted of decoction and maceration, with distilled water acting as the solvent for extraction. Bovine Serum Protein Denaturation was used as a method to evaluate the anti-inflammatory activity of S.vulgaris. Moreover, the evaluation of the hemolytic effect of aqueous extracts from S.vulgaris parts was prepared in vitro on an erythrocyte suspension of human blood incubated in phosphate buffer solution (PBS). The results of in-vitro anti-inflammatory activity showed that the macerated aqueous extract (Mac-H2O) and decocted aqueous extract (Dec-H2O) of leaves (at concentrations of 1 mg/ml) exhibited a considerably greater level of inhibition (88.31% and 66.88%, respectively) compared to the other parts. Additionally, an evaluation of hemolytic potential was conducted on human erythrocytes, revealing that the root extracts (Mac-H2O and Dec-H2O) display high activity against erythrocytes, leading to hemolysis (82.97 ± 3.56% and 86, 62 ± 1.44%, respectively). In contrast, the aerial parts showed very weak hemolytic activity. These results indicate that S.vulgaris has considerable antioxidant properties, particularly the leaves, which can make it a good nominee for subsequent investigations.
Keywords
Antiinflammatory activity; Decoction; Hemolytic activity; Maceration; Silene vulgaris
Statistics
Article View: 111
PDF Download: 44