Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis
El Naggar, H., Shehata, M., Abdelmaksoud, H., Barakat, A., Abdelhameed, R., Mohammad, O., El-Ashkar, A. (2024). Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis. EKB Journal Management System, 17(1), 37-45. doi: 10.21608/puj.2024.273767.1239
Heba El Naggar; Mai Shehata; Hagar Abdelmaksoud; Ashraf Barakat; Reda Abdelhameed; Omnia Mohammad; Ayman El-Ashkar. "Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis". EKB Journal Management System, 17, 1, 2024, 37-45. doi: 10.21608/puj.2024.273767.1239
El Naggar, H., Shehata, M., Abdelmaksoud, H., Barakat, A., Abdelhameed, R., Mohammad, O., El-Ashkar, A. (2024). 'Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis', EKB Journal Management System, 17(1), pp. 37-45. doi: 10.21608/puj.2024.273767.1239
El Naggar, H., Shehata, M., Abdelmaksoud, H., Barakat, A., Abdelhameed, R., Mohammad, O., El-Ashkar, A. Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis. EKB Journal Management System, 2024; 17(1): 37-45. doi: 10.21608/puj.2024.273767.1239

Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis

Parasitologists United Journal
Article 6, Volume 17, Issue 1, April 2024, Page 37-45  XML PDF (1.03 MB)
Document Type: Original Article
DOI: 10.21608/puj.2024.273767.1239
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Authors
Heba El Naggarorcid 1; Mai Shehata2; Hagar Abdelmaksoudorcid 3; Ashraf Barakat4; Reda Abdelhameed5; Omnia Mohammad6; Ayman El-Ashkar* 1
1Departments of Medical Parasitology , Faculty of Medicine, Ain Shams University , Cairo,
2Departments of Medical Parasitology1, Faculty of Medicine, Ain Shams University1, Cairo,
3Theodor Bilharz Research Institute
4Giza; Zoonotic Diseases, National Research Centre
5Applied Organic Chemistry, Chemical Industries Research Institute , Giza; Egypt
6Departments of Medical Parasitology , Faculty of Medicine, Ain Shams University
Abstract
Background: Since current therapies for toxoplasmosis are only effective against tachyzoites in acute
infections, and the occurrence of drug resistance, researches are directed to use natural products in the
treatment of chronic toxoplasmosis.
Objective: To investigate the potential efficacy of metal-organic framework (MOF) loaded coconut oil
(CO) and ginger oil (GO) nanoparticles (NPs) in the treatment of chronic murine toxoplasmosis.
Material and Methods: Ninety laboratory bred Swiss Albino mice were divided into 8 groups (10 mice
each); GI (negative control), GII (infected control), GIII-GVIII (infected with Me49 strain of T. gondii
and treated with MOFs-NPs, Spiramycin, Spiramycin loaded on MOFs-NPs, CO-MOF-NPs, GO-MOF-NPs,
and CO+GO-MOFs-NPs, respectively). Parameters used for evaluation included brain cyst count, tissue
pathology, and CD8+ infiltration of the liver.
Results: A statistically significant difference was observed in the number of brain cysts between all
infected groups receiving treatment, and GII; and GV showed the lowest count of brain cysts with a 60.8%
reduction. Histopathological examination showed that loading CO and GO separately or combined with
MOFs-NPs significantly restored the normal architecture of all examined tissues, i.e., brain, eye, liver, and
kidney. Using immunohistochemical (IHC) staining, high-density CD8+ infiltration was recorded in the
liver section of both GI and GII. While GIII, GIV, and GVI displayed low-density CD8+ infiltration, GV, GVII,
and GVIII showed intermediate-density.
Conclusion: Loading on MOFs-NPs, CO, and GO offered promising phytotherapy against chronic
toxoplasmosis.
Keywords
coconut oil; chronic toxoplasmosis; ginger oil; metal-organic frameworks; nanoparticles; spiramycin
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