Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls
Abdo, S., Abdelghany, E., Helal, M., Sakr, A., Abu El-Magd, M., Hegazy, M., EL-Kassas, S. (2024). Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls. EKB Journal Management System, 56(3), 501-510. doi: 10.21608/ejvs.2024.276609.1921
Safaa E. Abdo; Esraa S. Abdelghany; Mohamed Helal; A. M. Sakr; Mohammed Abu El-Magd; mohamed Hegazy; Seham EL-Kassas. "Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls". EKB Journal Management System, 56, 3, 2024, 501-510. doi: 10.21608/ejvs.2024.276609.1921
Abdo, S., Abdelghany, E., Helal, M., Sakr, A., Abu El-Magd, M., Hegazy, M., EL-Kassas, S. (2024). 'Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls', EKB Journal Management System, 56(3), pp. 501-510. doi: 10.21608/ejvs.2024.276609.1921
Abdo, S., Abdelghany, E., Helal, M., Sakr, A., Abu El-Magd, M., Hegazy, M., EL-Kassas, S. Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls. EKB Journal Management System, 2024; 56(3): 501-510. doi: 10.21608/ejvs.2024.276609.1921

Association of Polymorphism in Leptin Gene with Semen Quality Parameters in Egyptian Buffaloes Bulls

Egyptian Journal of Veterinary Sciences
Volume 56, Issue 3, March 2025, Page 501-510  XML PDF (1.28 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.276609.1921
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Authors
Safaa E. Abdo1; Esraa S. Abdelghany2; Mohamed Helal3; A. M. Sakr4; Mohammed Abu El-Magd5; mohamed Hegazyorcid 6; Seham EL-Kassas email orcid 7
1Genetics and Genetic Engineering, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.
2Genetics and Genetic Engineering, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
3Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
4Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
5Department of Anatomy& Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
6Department of Biotechnology, Animal Production Research Institute, Egypt
7Animal, Poultry and Fish Breeding and Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
Abstract
This study aimed to identify the single nucleotide polymorphisms (SNPs) in a partial sequence of the leptin gene and its potential association with the semen quality parameters and mRNA expression levels of the leptin gene in the Egyptian buffalo' bulls. A total of 20 Egyptian buffalo bulls were used in this study. The selected amplicon of the leptin gene was amplified using a specific primer. After that, the SNPs detection and genotyping were performed based on sequence analysis and high-resolution melting (HRM)-PCR. A c.216A>G synonymous SNP (g.14328A>G) was identified in the assessed partial leptin sequence. This SNP showed significant linkages with the semen profile. In this regard, the heterozygous AG genotype exhibited significantly higher progressive motility (PR), sperm concentration, total sperm count, and higher LH and testosterone hormone levels. While the GG genotype showed higher normal morphology form % and functional sperm %. Moreover, AG and GG genotypes displayed significant upregulation of leptin mRNA levels compared to the AA genotype. The g.14328A>G SNP could be considered an effective marker in marker-assisted selection and breeding programs for improving the fertility of Egyptian buffalo bulls. 
Keywords
Leptin; Egyptian buffalo; SNP; HRM-PCR; fertility
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