Emergence of New Delhi Metallo Beta Lactamase blaNDM-1 and Oxacillinases blaOXA-48 Producing Klebsiella pneumoniae in an Egyptian Hospital
Shawky, A., Tolba, S., Hamouda, H. (2019). Emergence of New Delhi Metallo Beta Lactamase blaNDM-1 and Oxacillinases blaOXA-48 Producing Klebsiella pneumoniae in an Egyptian Hospital. EKB Journal Management System, 54(1), 25-37. doi: 10.21608/ejm.2019.12395.1096
Amal M. Shawky; Sahar T.M. Tolba; Hayam M. Hamouda. "Emergence of New Delhi Metallo Beta Lactamase blaNDM-1 and Oxacillinases blaOXA-48 Producing Klebsiella pneumoniae in an Egyptian Hospital". EKB Journal Management System, 54, 1, 2019, 25-37. doi: 10.21608/ejm.2019.12395.1096
Shawky, A., Tolba, S., Hamouda, H. (2019). 'Emergence of New Delhi Metallo Beta Lactamase blaNDM-1 and Oxacillinases blaOXA-48 Producing Klebsiella pneumoniae in an Egyptian Hospital', EKB Journal Management System, 54(1), pp. 25-37. doi: 10.21608/ejm.2019.12395.1096
Shawky, A., Tolba, S., Hamouda, H. Emergence of New Delhi Metallo Beta Lactamase blaNDM-1 and Oxacillinases blaOXA-48 Producing Klebsiella pneumoniae in an Egyptian Hospital. EKB Journal Management System, 2019; 54(1): 25-37. doi: 10.21608/ejm.2019.12395.1096

Emergence of New Delhi Metallo Beta Lactamase blaNDM-1 and Oxacillinases blaOXA-48 Producing Klebsiella pneumoniae in an Egyptian Hospital

Egyptian Journal of Microbiology
Article 3, Volume 54, Issue 1, 2019, Page 25-37  XML PDF (1.57 MB)
Document Type: Original Article
DOI: 10.21608/ejm.2019.12395.1096
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Authors
Amal M. Shawky1, 2; Sahar T.M. Tolba email 1; Hayam M. Hamouda2
1Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
2Microbiology Department , National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Abstract
The rapid dissemination of carbapenem resistant Enterobacteriaceae (CRE) all over the world represents a matter of concern. This study aims to investigate the prevalence of CRE in clinical isolates recovered from Al Kasr Al-Ainy hospital in the period between August 2015 and February 2017. The isolates were identified by conventional methods and Maldi-TOF spectroscopy. Phenotypic identification of CRE was carried out by Modified Hodge test and genotypic characterization for Extended-Spectrum Beta-Lactamase (ESBL) and CRE genes was also performed. Carbapenemase activity of CRE isolates was confirmed in 46% of the isolates of which, blaNDM-1 and blaOXA-48 were detected in 75%, 59% of the isolates respectively, while blaVIM was detected in 2.3% only. However, blaIMP and blaKPC were not detected in any isolate. All CRE isolates carried at least one ESBL gene, 95.4% of CRE isolates had blaCTX-M-15, 88.6% blaTEM-1, and 68.2% blaSHV. The blaSHV gene showed different alleles in the CRE isolates. All the isolates were sensitive to polymyxin B and colistin while only 36.4% were sensitive to tigecycline. Consequently, microbiologists and clinicians should implement the necessary control measures to prevent the spreading of these resistant bacteria.
Keywords
<i> Klebsiella pneumoniae </i>; Carbapenem resistance; blaNDM-1; blaOXA-48; ESBLs; MHT
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