Effect of GnRH Administration at Mating and During Luteal Phase on Pregnancy Rate of Repeat Breeder Buffalo Cows | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 63, Issue 1, January 2017, Page 65-74 PDF (6.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2017.354951 | ||||
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| Authors | ||||
| M.A Abo-Farw* ; M.M Ghoneim | ||||
| Animal Production Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| Abstract | ||||
| To evaluate the potentiality of GnRH agonist injection, on day 0,11 or 13 post-mating on pregnancy rate of repeat breeder buffalo cows, total of 40 cyclic Egyptian buffalo cows (4-7 years and 400-500Kg weight) were i.m. injected with 2.5ml PGF2α to synchronize estrus. Animals in heat (within 72h) were naturally mated and allotted randomly to four groups (10 in each). Animals in the 1st, 2nd and 3rd groups were i.m. injected with 205ml GnRH on day 0(G1),11(G2) and 13 (G3) of estrus onset, respectively, while G4 control injected with 2.5 ml saline. Blood samples were collected from jugular vein on day 0, 3, 5, 7,9, 11. 13.15 17 19, 21, 23 and 25 of estrus. Pregnancy was diagnosed day 25 post-mating of each animal using ultrasound examination. Results showed that serum P4 concentration increased (P<0.05) following GnRH injection up to day 13,17 and 25 day post-mating in G1, G2 and G3 as compared to G4, respectively. Serum P4 was the highest (P<0.05) in G3 from day 17 to 25 post mating, being different (P<0.05) from G4, but dud not differ than that in G1 and G2. Pregnancy rat was 70,70,80 and 50% in G1, G2, G3 and G4, respectively (P<0.05). Concentration of P4 was higher (P<0 05) in pregnant than in non-pregnant buffalo as overall mean for all groups or within each experimental group, being the highest in G3. Therefore, this study may conclude that injection of GnRH analogue (2.5 ml Receptal/animal) on day 13 post-mating improved serum progesterone concentration and pregnancy rate of repeat breeder buffalo cows as overall mean for all groups or within each experimental group compared to those injected on day 0 or 11 post-mating. | ||||
| Keywords | ||||
| Buffalo; Repeat breeder; GnRH; Estrous; Luteal phase; Progesterone; Pregnancy | ||||
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