Effects of Aqueous and Ethanolic Extracts of Ginger (Zingiber Officinale) Rhizome on Serum Progesterone Level and Markers of Oxidative Stress in African Giant Rat (Cricetomys Gambianus) in Captivity | ||||
| Journal of Applied Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 18 May 2024 PDF (371.32 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2024.278828.1326 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
FODA F.C.  1; MINGOAS K.J.P.2; CHONGSI M.M.M.3; FONOU T.L.4; TAKAM B.B.I.3; FONKEM S.3; VEMO B.N.4; KENFACK A.3
| ||||
| 1Department of Animal Science, Faculty of Agronomy and Agricultural Science, University of Dschang, West, Cameroon. | ||||
| 2Department of Physiology and Biochemistry, School of Sciences and Veterinary Medicine, University of Ngaoundéré, Cameroon | ||||
| 3Department of Animal Science, Faculty of Agronomy and Agricultural Science, University of Dschang, Cameroon | ||||
| 4Department of Animal Science, Faculty of Agriculture and Veterinary Medicine, University of Buea, Cameroon | ||||
| Abstract | ||||
| The African giant rat is a very prolific animal in its natural biotope. But in captivity, this species exhibits poor reproductive performance due to embryonic resorption. These resorptions are due to oxidative stress or a lack of progesterone production. This study was carried out to evaluate whether aqueous and ethanolic extracts of ginger, given their antioxidant and steroidogenic properties, can improve the antioxidant status and serum progesterone level during gestation in African giant rats. For this purpose, twenty-eight adult female African giant rats were divided into seven treatments comparable in terms of body weight: the control group received only avocado paste (7 g/day), while the other groups received either aqueous or ethanolic extracts at doses of 250, 500, and 750 mg/kg bw in a homogeneous mixture of paste. Animals were individually housed and had free access to food and drinking water. Eight days after the start of the test, the females were bred with males. Each female was sacrificed on the 15th day post-coitus, then the blood was collected from the jugular vein and centrifuged at 3500 rpm for 15 minutes and the serum obtained was used for the determination of progesterone and markers of oxidative stress concentrations. The results showed that except for females who had received 750 mg/kg bw of aqueous extract, the serum progesterone level was significantly (P˂0.05) higher in the treated animals compared to the control. The administration of the ethanolic extract at doses of 250 and 500 mg/kg bw induced a significant (P˂0.05) increase in the levels of malondialdehyde, catalase and superoxide dismutase compared to the control group. On the other hand, the administration of aqueous extract significantly (p < 0.05) lowered the level of total peroxidases compared to the control. Aqueous and ethanolic extracts of ginger can be used to increase serum progesterone levels, but cannot be used to improve oxidative stress status. | ||||
| Keywords | ||||
| African giant rat; Ginger; Oxidative stress; Progesterone; Reproduction | ||||
| References | ||||
|
ALAAELDIN, A.H., GEHAM, H.H., SALSABIL, H., ALI, A., and AMR, A., 2021. Standardized extract of ginger ameliorates liver cancer by reducing proliferation and inducing apoptosis through inhibition oxidative stress/ inflammation pathway. Journal of medicine and Pharmacotherapy 134, 111102. . https://doi.org/10.1016/j.biopha.2020.111102.
AMIR, M.A.H., MOHAMMAD, H.A., REHAM, D.A.,AYMAN.M.M., 2018. Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy. Biomedicine & Pharmacotherapy 106, 381-389. https://doi.org/10.1016/j.biopha.2018.06.148.
AUROUSSEAU, B., DURAND, D., and GRUFFAT, D., 2004. Contrôle des phénomènes oxydatifs pendant la gestation chez les monogastriques et les ruminants. NRA Productions. Animales 17(5), 339-354. https://doi.org/10.20870/productions-animales.2004.17.5.3607.
BAKKALI, F., AVERBECK, S., AVERBECK, D., and IDAOMAR, M., 2008. Biological effects of essential oils. Food and Chemical Toxicology46, 446-475. https://doi.org/10.1016/j.fct.2007.09.106.
BUSMAN, H., KANEDI, M., 2016. Testicular Function of Rats Treated with Water Extract of Red Ginger (Zingiber officinale var rubrum) Combined with Zinc. Journal of Food and Nutrition Research 4(3), 157-162.https://doi.org/10.12691/jfnr-4-3-5.
DONGMO, N.A.B., VEMO, B.N., TCHOFFO, H., LONTIO, F.A., MENKEM, B., DJUISSI, M.N., MOHAMADOU, A., MAHAMAT, T.M.A., CHONGSI, M.M.M., and NGOULA, F., 2021. Oxidative status and reproductive characteristics in female cavies (Cavia porcellus l.) Fed on aqueous extract of avocado (persea americana mill.) Seed. Journal of Veterinary Healthcare 2(3), 17-28. https://doi.org/10.14302/issn.2575-1212.jvhc-21-3759.
FLAVIEN, B., 2023. Physiologie de la grossesse Pregnancy physiology. Actualités Pharmaceutiques 62(628), 18-21. https://doi.org/10.1016/j.actpha.2023.06.005.
FONKEM, S., TSAMBOU, M.A.M., FONOU, T.L., FODA, F.C., VEMO, B.N., TAKAM, M.B.I., KOUAMO, J., and KENFACK, A., 2022. 17-α-hydroxyprogesterone caproate improves female African giant rats (Cricetomys gambianus) fertility in captivity. Journal of Agriculture and Veterinary Science15(3), 15-21. https://doi.org/10.9790/2380-1503031521.
GHOLAMI-AHANGARAN, M., KARIMI-DEHKORDI, M., JAVAR, A.A., SALEHI, M.H., and OSTADPOOR, M., 2021. A systematic review on the effect of Ginger (Zingiber officinale) on improvement of biological and fertility indices of sperm in laboratory animals, poultry and humans. Veterinary Medicine and Science 7(5), 1959‑1969. https://doi.org/10.1002/vms3.538.
GUTTERIDGE, J., and HALLIWELL, B., 2000. Free radicals and antioxidants in the year 2000: a historical look to the future. Annals of the New York Academy of Sciences 899(1), 136-147. https://doi.org/10.1111/j.1749-6632.2000.tb06182.x.
HABBU, P.V., SHASTRY, R.A., MAHADEVAN, K.M., HANUMANTHACHAR, J., and DAS, S.K., 2008. Hepatoprotective and antioxidant effects of Argyreia speciosa in rats. African Journal of Traditional Complementary and Alternative Medicine 5(2), 158-164.https://doi.org/10.4314/ajtcam.v5i2.31268.
KAÏS, H.A-G., PAUL, A.F., and CATHERINE, G., 2010. The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes. International Journal of Biochemistry and Cell Biology 42(10) 1634-1650. https://doi.org/10.1016/j.biocel.2010.06.001.
MISRA, H.P., and FRIDOVICH, I., 1972. The generation of superoxide radical during the autoxidation of hemoglobin. Journal of Biological Chemistry 247(21), 6960-6962.https://doi.org/10.1016/S0021-9258(19)44679-6.
MUSA, Y., MUSBAU, A.A., TEMIDAYO, O., 2006. Aphrodisiac potential of the extract of Fadogia agrestis (Scweinf Ex Hierm) stem in male albino rats. ResearchGate 7(4), 399-404. https://doi.org/10.1111/j.1745-7262.2005.00052.x.
MUTINATI, M., PICCINNO, M., RONCETTI, M., CAMPANILE, D., RIZZO, A., and SCIORSCI, R.L., 2013. Oxidative stress during pregnancy in the sheep. https://doi.org/10.1111/rda.12141.
NAFIYE, Y. 2018. Ginger (Zingiber officinale) might improve female fertility: A rat model. Journal of the Chinese Medical Association 81, 905-911.https://doi.org/10.1016/j.jcma.2017.12.009.
NILSSON, U.A., OLSSON, L.I., CARLIN, G., and BYLUND-FELLENIUS, A.C., 1989. Inhibition of lipid peroxidation by spin labels, Journal of Biological Chemistry 264(19), 11131-11135. https://doi.org/10.1016/S0021-9258 (18)60439-9.
SHANMUGAM, M., KOROVI, M., NISHANTH, K. and KERSIREDDY, S.R., 2011. Neuroprotective effect of ginger on anti-oxidant enzymes in streptozotocin-induced diabetic rats. Food and Chemical Toxicology 49(4), 893-897. https://doi.org/10.1016/j.fct.2010.12.013.
SINHA, A.K. 1972. Colorimetric assay of catalase. Analytical Biochemistry 47(2), 389-394. https://doi.org/ 10.1016/0003-2697(72)90132-7.
SIROTKIN, A.V., KODAS, A., STOCHR, A., BALAZI, A., FOLDES, M., MAKOV, P., CHRENE, P., and HARRATH, A.H., 2018. Effect of turmeric on the viability, ovarian folliculogenesis, ovarian hormones and reponse to luteinizing of rabbits. Animal12, 1242-1249. https://doi.org/10.1017/S175173111700235X.
STENQVIST, A., OLESZCZUK, K., LEIJONHUFVUD, I.1., GIWERCMAN, A., 2018. Impact of antioxidant treatment on DNA fragmentation index: a double-blind placebo-controlled randomized trial. Andrology 6(6), 811-816. https://doi.org/10.1111/andr.12547.
STEPHANIA, T., 2015. Physiology, production and action of progesterone. Reproductive Medicine Unit, GynePro Medical Group, Bologna, Italy 94, 8-16. https://doi.org/10.1111/aogs.12771.
TAPSELL, L.C., HEMPHILL, I., COBIAC, L., PATCH, C.S., SULLIVAN, D.R., FENECH, M., and INGE, K.E., 2006. Health benefits of herbs and spices: The past, the present, the future.Australian Medical Research 185, 4-24. https://doi.org/10.5694/j.1326-5377.2006.tb00548.
TCHOFFO, H., KANA, J.R., NGOULA, F., FOLACK, T.L.V., ADOUM, G., MOUSSA, M.O., and NGOUOZEU, M.W.M., 2018. Growth perfomance, serum biochimical profile, oxidative status and fertility traits in male japanese quail fed on ginger (Zingiber officinale roscoe) essential oil. University of Dschang. Veterinary Medicine International.7682060.https://doi.org/10.1155/2018/7682060.
YAKUBU, M.T., and AKANJI, M.A., 2011. Effect of aqueous extract of Massularia acuminate stem on sexual behaviour of male Wistar rats. Evidence-Based Complementary and Alternative Medicine. 10 pp. https://doi.org/10.1155/2011/738103.
ZHAO, X., YANG Z.B., YANG, W.R., WANG, Y., JIANG, S.Z., and ZHANG, G.G., 2011. Effects of ginger roots (Zingiber officinale) on laying performance and antioxidant status of laying hens and on dietary oxidation stability. Poultry Science 90, 1720-1727. https://doi.org/10.3382/ps.2010-01280.
| ||||
|
Statistics Article View: 54 PDF Download: 41 |
||||
