Phytochemical Analysis and Therapeutic Evaluation of Ehretia anacua Against Streptozotocin-Induced Diabetes in Rats | ||||
| Egyptian Journal of Chemistry | ||||
| Articles in Press, Accepted Manuscript, Available Online from 19 May 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.282176.9586 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mahmoud R. Hussein 1; Mohamed E. Abouelela 2; Reda A. Abdelhamid  1; Khalid S. Hassanin3; Lotfy D. Ismail2
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| 1Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Assiut 71524, Egypt. | ||||
| 2Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt. | ||||
| 3Department of pharmacology and toxicology, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt. | ||||
| Abstract | ||||
| Diabetic patients have a higher risk of health problems, including heart attack, stroke, kidney failure, and permanent vision loss. Ehretia anacua is a plant used in folk medicine for the management of diabetes. The current study investigated the phytochemical profile of Ehretia anacua and evaluated its antioxidant activity, acute toxicity, and therapeutic potential against streptozotocin-induced diabetes in a rat model. Additionally, we evaluated the phenolic and flavonoid content either colorimetrically or by HPLC analysis. Eleven compounds were isolated namely, phytyl palmitate (1), β-sitosterol tetracosanoate (2), palmitic acid (3), isophytol (4), β-sitosterol (5), β-sitosterol-3-O-β-D-glucopyranoside (6), methyl rosmarinate (7), caffeic acid (8), (6R,7E,9R)-9-hydroxy-megastigma-4,7-dien-3-one 9-O-D-glucopyranoside (9), roseoside (10) and rosmarinic acid (11). These results revealed that E. anacua is a rich source of phenolic compounds. The ethyl acetate and n-butanol fractions have potent antioxidant activity with value of IC50 is 25.16 ± 0.09 and 87.84 ± 0.39 µg/mL, respectively. Moreover, the ethyl acetate fraction exhibited superior antidiabetic efficacy by significantly reducing blood sugar levels and lipid profiles and modulating related biochemical parameters. These results suggest that E. anacua leaves could serve as a potential source of antidiabetic and antioxidant medications, possibly offering new avenues for combination therapy for diabetes treatment. | ||||
| Keywords | ||||
| Ehretia anacua; antidiabetic; antioxidant; phytochemical analysis; total phenolic; total flavonoids; HPLC | ||||
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