In Vitro Study of the Effect of Cypermethrin on Human Sperm Function, DNA Fragmentation and the Possible Protective Role of Vitamins C and/ or E | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 12, Volume 32, Issue 1, June 2012, Page 173-189 PDF (261.84 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2012.35582 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Adel Zalata* 1; Hussein Abdalla1; Mohamed Serria1; Amal Aziz2; Sahar El-Dakroory3; Amal El-Bakary3 | ||||
| 1Medical Biochemistry, Faculty of Medicine, Mansoura University | ||||
| 2Clinical Pathology Dept. Faculty of Medicine, Cairo University | ||||
| 3Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Mansoura University, Egypt | ||||
| Abstract | ||||
| Pyrethroid pesticides are used preferably over organochlorines and organophosphates due to their high effectiveness, low toxicity to non-target organisms and easy biodegradability. Cypermethrin, a type II synthetic pyrethroid pesticide, is widely used in Egypt in pest control programs in agriculture and in public health as well. The objective of the present study was to evaluate the potential of cypermethrin genotoxicity in sperm and to investigate the possible ameliorative effects of vitamin C and/or E on cypermethrin toxicity. This study was done on semen samples collected from 10 healthy normozoospermic volunteers. Each semen sample was divided into six aliquots. One served as control negative aliquot (group I) that was not exposed to any treatments. The second aliquot (group II) was incubated with 20 mM vitamin C (ascorbic acid) and 2 mM vitamin E (α-tocopherol). The third aliquot was exposed to cypermethrin with a dose of 10 μM (group III) for 6 hours at 37°C, while the other three aliquots (IV, V, VI) were incubated with 20 mM vitamin C, 2 mM vitamin E and vitamin C & E (20 mM, 2 mM) respectively for 30 min before cypermethrin exposure. All aliquots were kept at room temperature. Unexposed and exposed aliquots were analyzed for sperm concentration, motility, and viability according to WHO guidelines. Hypo-osmotic Swelling (HOS) test and the modified alkaline comet assay were carried out on the prepared samples. There was statistically significant decrease in parameters of sperm motion, seminal functions and increase in sperm DNA damage parameters in cypermethrin group. With addition of antioxidant vitamins C and E either alone or combined there was statistically significant improvement in all of the parameters of sperm motion, seminal functions and DNA damage parameters and the maximal improvement was with the combined vitamin C and E. It could be concluded that cypermethrin can alter sperm function and induce genotoxic effect on sperms in vitro and that the antioxidant vitamins (C and E) might be useful in antagonizing the toxic effects of cypermethrin on sperm. | ||||
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