Detection of type I collagen fragments in the gingival crevicular fluid and saliva during orthodontic tooth movement
Saud, O., Tawfik, N., Enany, N., Kassem, H. (2024). Detection of type I collagen fragments in the gingival crevicular fluid and saliva during orthodontic tooth movement. EKB Journal Management System, 65(1), 1-19. doi: 10.21608/eos.2023.240588.1090
Omar Tareq Saud; Nourane Tawfik; Nagwa Enany; Hassan Kassem. "Detection of type I collagen fragments in the gingival crevicular fluid and saliva during orthodontic tooth movement". EKB Journal Management System, 65, 1, 2024, 1-19. doi: 10.21608/eos.2023.240588.1090
Saud, O., Tawfik, N., Enany, N., Kassem, H. (2024). 'Detection of type I collagen fragments in the gingival crevicular fluid and saliva during orthodontic tooth movement', EKB Journal Management System, 65(1), pp. 1-19. doi: 10.21608/eos.2023.240588.1090
Saud, O., Tawfik, N., Enany, N., Kassem, H. Detection of type I collagen fragments in the gingival crevicular fluid and saliva during orthodontic tooth movement. EKB Journal Management System, 2024; 65(1): 1-19. doi: 10.21608/eos.2023.240588.1090

Detection of type I collagen fragments in the gingival crevicular fluid and saliva during orthodontic tooth movement

Egyptian Orthodontic Journal
Volume 65, Issue 1, June 2024, Page 1-19  XML PDF (1.04 MB)
Document Type: Original Article
DOI: 10.21608/eos.2023.240588.1090
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Authors
Omar Tareq Saud email orcid 1; Nourane Tawfik2; Nagwa Enany3; Hassan Kassem4
1Orthodontics Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
2Lecturer, Clinical Pathology, Faculty of Medicine , Alexandria University, Alexandria, Egypt.
3Professor, Department of Orthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
4Department of Orthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Abstract
Introduction: The purpose of this study was to investigate the levels of Type I Collagen fragments ICTP (cross-linked carboxyterminal telopeptide of type I collagen) and CTX (C-terminal cross-linked telopeptide of type I collagen) in gingival crevicular fluid (GCF) and saliva in the leveling and alignment phase of orthodontic tooth movement.
Materials and methods: Fourteen adolescent patients [5 males, 9 females; mean age = 15.5 yrs (range = 13.1 - 17.6)] with moderate mandibular incisor crowding were fitted with preadjusted edgewise brackets and 0.014 nickel-titanium (NiTi) wires. The main outcome measure was the levels of CTX and ICTP in the GCF and saliva. Samples were obtained from lower anterior teeth at 0, 1, 3, 7, 14, and 21 days after appliance placement. Enzyme-linked immunosorbent assay analysis (ELISA) was used to measure the levels of ICTP and CTX in the collected samples.
Results: GCF and saliva ICTP and CTX levels did not significantly change between the time points. A statistically significant positive correlation and agreement was found between the levels of ICTP and CTX in saliva (P= 0.0001). However, there was no statistically significant correlation between the GCF and salivary levels of either ICTP or CTX or between ICTP and CTX levels in GCF. The CTX saliva level was not a statistically significant predictor of ICTP in saliva (p = 0.986).
Conclusion: Changes in ICTP and CTX levels in human GCF and saliva cannot be used as biological biomarkers during the initial stage of orthodontic tooth movement in adolescent patients.
Keywords
Orthodontic tooth movement; ICTP; CTX; Gingival crevicular fluid (GCF); Saliva
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