IMPACT OF Allium sativum AGAINST Enterobacter sp. AS WATER BORNE PATHGENIC BACTERIA ISOLATED FROM RIVER NILE | ||||
| Arab Universities Journal of Agricultural Sciences | ||||
| Article 36, Volume 26, Special issue (2D) - Serial Number 6, February 2018, Page 2525-2531 PDF (874.18 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajs.2018.35621 | ||||
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| Authors | ||||
| Rasha A. Ebrahim1; Rawia F. Gamal2; Samah H.A. Mohamed2; R. Z. Abdel-Rahman3 | ||||
| 1Medical Analysis Central Laboratories for Medical Analysis and Blood Bank of Armed Forces, Al-Khalifa Al-Ma'mun, Cairo, Egypt | ||||
| 2Agric. Microbiology Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, Egypt | ||||
| 3Medical Microbiology Dept., Military Medical Academy, Al-Khalifa Al-Mamun, Cairo, Egypt | ||||
| Abstract | ||||
| To explore the antibacterial activities of Allium sativium (garlic) extract was tested against two waterborne pathogenic strains isolated from River Nile, to mitigate the increase of bacterial resistance to conventional antibiotics. The two isolates were identified as Enterobacter cloacae DSM 3264 BRB & Enterobacter cloacae MB11506_1CHB by MALDI-Tof-MS. Aqueous, methanolic and oil extraction of garlic were tested for their inhibitory activity against the selected strains using well diffusion method. Enterobacter sp. were more sensitive towards oil extract with inhibition zone 2.3 cm rather than aqueous and methanolic extractions with was 50%. Analysis of garlic essential oil by GC-MS dedicated six sulfur compounds represented 25% of total detected compounds in the oil. | ||||
| Keywords | ||||
| Enterobacter cloacae; garlic oil; MALDI-TOF-MS; GC-MS spectrometry. Allium sativum; Well diffusion method | ||||
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