Changes in Urinary Bladder Smooth Muscle Contractility and the Role of Rho Kinase and Isoprostane after Partial Bladder outlet Obstruction in rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 1, Volume 32, Issue 2, December 2012, Page 1-16 PDF (228.43 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2012.35784 | ||||
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| Authors | ||||
| Abeer Dief* 1; Adham Mohamed1; Gehan Sharara2 | ||||
| 1Department of Medical Physiology Faculty of Medicine-Alexandria University | ||||
| 2Department of Biochemistry Faculty of Medicine-Alexandria University | ||||
| Abstract | ||||
| Objective: Bladder outlet obstruction is a common urological problem that associated with many cellular changes and remodeling of urinary bladder detrusor smooth muscle. This study was designed to determine the changes in Rho kinase (ROCK) and isoprostane levels in urinary bladder homogenate after partial bladder outlet obstruction (PBOO); and to evaluate the contractile response of isolated rat detrusor muscle after PBOO. Methods: The present work was carried out on 30 male albino rats that were divided into 3 groups (10 rats/group): group I: consisted of normal rats, they were sham operated and considered as control group, group II: consisted of rats that were undergone PBOO for 14days, group III: consisted of rats that were undergone PBOO for 28 days. The following parameters were assessed in rats of all groups: urinary bladder weight, levels of both Rho kinase and isoprostane in the tissues of urinary bladder as well as the contractile response of detrusor muscle both spontaneously and in response to acetyl choline. Results: The present study revealed a significant increase in the weight of urinary bladder in rats of PBOO of group II and III as compared to sham operated control of group I. There was a significant increase in the mean value of Rho kinase and isoprostane in the tissue of the urinary bladder after obstruction. 14 days after obstruction was associated with a significant increase in the mean value of Rho kinase which was significantly high compared to group I (sham operated rats) and group III (28days of obstruction), 28 days of obstruction was associated with decrease in the mean value of Rho kinase compared to 14 days of obstruction, however, its value was above the control. Regarding the contractile response, PBOO was associated with increased spontaneous activity of detrusor muscle and increased tonic response to acetyl choline which was significantly high after 14 and 28 days of obstruction compared to the control. Conclusion: The results of the present study demonstrated that Rho kinase and isoprostane levels were elevated after bladder outlet obstruction and that Rho kinase could play a crucial role in the regulation of rat urinary bladder smooth muscle contraction and tone. Therefore, Rho kinase inhibitors may prove to be therapeutically useful in the treatment of lower urinary tract disorders associated with changes in the physiology of bladder smooth muscle contractility. | ||||
| Keywords | ||||
| Urinary bladder-Obstruction- Contractility- Rho kinase- Isoprostane | ||||
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