EFFECT OF ACUTE MORPHINE ADMINISTRATION ON HORMONAL MODULATION AND THEIR RELATION TO MALE REPRODUCTION | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 34, Volume 76, Issue 3, September 1998, Page 1285-1295 PDF (2.78 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.1998.359106 | ||||
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| Authors | ||||
| MAGED A. F. EL-SHELTAWI; SAYED A. ESSAWY; GERGES A. BEDIR | ||||
| Animal Health Research Institute, Agricultural Research Centre, Giza, Egypt | ||||
| Abstract | ||||
| Twenty-four adult male Sprague-Dawley rats weighing 130-140 grams were used in this study. In experiment I. twelve intact adult sexually active male rats.were sub-divided into two equal groups. In group A, six animals were injected with 0.2m1 saline 60 min. before experimentation, while, in group 6, animals were injected with 10mg/kg. b. w morphine sulphate 60 min. before experimentation. In experiment II, twelve male rats were used to study the effect of acute morphine administration in castrated male rat (castrated two weeks earlier). Animals were subdivided into two equal groups. In group A, animals were injected with 0.2 ml saline 60 min. before starting the experimentation, while, in group B, animals were injected with 10 mg/kg b. w. morphine sulphate 60 min. before experimentation. In both experiments, exploratory behaviour parameters (sniffing, rearing and investigating movement) were recorded for both latency and frequency, sexual behaviour parameters (mounting, intromission and ejaculation) were also recorded. At the end of 30 minute-behavioural test, all animals were sacrificed and trunk blood samples were collected. LH and FSH and prolactine were assayed in blood serum. Epididymal spermatozoa were collected and examined for spem concentration (million / ml) sperm viability (%), sperm motility (%) and abnormal forms (%). The obtained results indicated that, in experiment I, acute morphine administration resulted in a non-significant (P<0.05) changes in all of the exploratory behaviour, inhibition in all parameters of sexual behaviour, significant (P<0.01) decrease in LH and FSH, and significant (P<0.01) increase in prolactin level. There was a significant (P<0.01) decrease in sperm viability and motility, while, there were non-significant changes in sperm concentration and abnormal forms. In experiment acute morphine administration in castrated male rats resulted in a nonsignificant change on the exploratory behaviour; a complete inhibition of the sexual behaviour, and non-significant change in LH, FSH and prolactin level. It is concluded that, acute morphine administration affects significantly male reproduction, while, castration does not enhance the effect of morphine administration. | ||||
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