Potent Effect of Superoxide Dismutase -Like Activity of Partially Purified Protein from Raphnus Sativus Roots on Transforming Growth Factor and Vascular Endothelial Growth Factor In Ehrlich Ascites Carcinoma Bearing Mice | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 22, Volume 32, Issue 2, December 2012, Page 309-326 PDF (317.99 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2012.35939 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Salem Habib* 1; Laila Eissa2; Mohammed Abdel Latif1 | ||||
| 1Chemistry Department (Biochemistry Division), Faculty of Science, Damietta University, New Damietta, Egypt | ||||
| 2Biochemistry Department, Faculty of Pharmacy, Mansoura University, Egypt | ||||
| Abstract | ||||
| The alterations in cancer cell's metabolism have been associated with enhanced oxidative stress. Therefore, a partially purified SOD-like activity protein from the roots of Raphnus sativus (R. sativus) and low-molecular-weight heparin (LMWH) were tested for their abilities to inhibit the growth of Ehrlich ascites carcinoma cells (EAC) intraperitoneally implanted in albino mice. Ninty mice were randomly divided into 6 groups. The antitumor effect of the partially purified protein and LMWH were assessed by estimating transforming growth factor Beta (TGF-β) and vascular endothelial growth factor (VEGF) in serum. Also, tumor volume, median survival time (MST), total lipids, DNA and RNA contents in liver tissues as well as liver function tests and the redox status were estimated. TGF-β, VEGF, DNA, RNA, and malondialdehyde (MDA) levels in addition to serum alanine transaminase (ALT) and gamma glutamyl transferase (GGT) activities and tumor volume were highly significantly increased (P<0.001) in the untreated EAC-bearing mice compared to those of the control. However, total lipids in liver tissues and serum albumin were highly significantly decreased in the same group compared to the corresponding values of the control group. All these parameters were restored to their normal levels in the partially purified extract and LMWH treated EAC-bearing mice groups compared to the untreated EAC-bearing mice. It could be concluded that, the partial purified protein with SOD-like activity from the roots of R. sativus and the LMWH have a remarkable anti-tumor activity against EAC cells in Swiss albino mice through its potent effect on both TGF-β and VEGF. | ||||
| Keywords | ||||
| Transforming growth factor-β; vascular endothelial growth factor; Ehrlich Ascites Carcinoma (EAC); Low-molecular-weight heparins; Raphnus sativus; and SOD-like activity | ||||
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